Skip to main content
Log in

Growth and characterisation of human faecal astrovirus in a continuous cell line

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

We report conditions for the growth of human faecal astrovirus in a continuous colonic carcinoma cell line (CaCo-2). Purified particles contained three polypeptides, one of which (24k) appeared loosely held on the exterior.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Fogh J, Trempe G (1975) New human tumor cell lines. In: Fogh J (ed) Human tumor cells in vitro. Plenum, New York, pp 115–159

    Google Scholar 

  2. Grasset E, Bernabeu J, Pinto M (1985) Epithelial properties of human colonic carcinoma cell line CaCo-2: effect of secretagogues. Am J Physiol 248: 410–418

    Google Scholar 

  3. Gray EW, Angus KW, Snodgrass DR (1980) Ultra structure of the small intestine in astrovirus infected lambs. J Gen Virol 49: 71–82

    Google Scholar 

  4. Harbour DA, Ashley CR, Williams PD, Gruffydd-Jones TJ (1987) Natural and experimental astrovirus infection of cats. Vet Rec 120: 555–557

    Google Scholar 

  5. Herring AJ, Gray EW, Snodgrass DR (1981) Purification and characterization of ovine astrovirus. J Gen Virol 53: 47–55

    Google Scholar 

  6. Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver stained polyacrylamide gels. J Clin Microbiol 16: 473–477

    Google Scholar 

  7. Hoshino Y, Zimmer JF, Moise NS, Scott FW (1981) Detection of astrovirus in faeces of a cat with diarrhoea. Arch Virol 70: 373–376

    Google Scholar 

  8. Kurtz JB (1988) Astroviruses. In: Farthing MJG (ed) Viruses and the gut. Proceedings of the Ninth BSG.SK and F International Workshop, Windsor, Berks, UK, 2–4 October, pp 84–87

  9. Kurtz JB, Lee TW (1984) Human astrovirus serotypes. Lancet ii: 1405

    Google Scholar 

  10. Kurtz JB, Lee TW (1987) Astroviruses: human and animal. In: Block G, Whelan J (eds) Novel diarrhoea viruses. Wiley, Chichester, pp 92–107 (Ciba Symposium 128)

    Google Scholar 

  11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227: 680–685

    Google Scholar 

  12. Lee TW, Kurtz JB (1981) Serial propagation of astrovirus in tissue culture with the aid of trypsin. J Gen Virol 57: 421–424

    Google Scholar 

  13. Lee TW, Kurtz JB (1982) Human astrovirus serotypes. J Hyg (Camb) 89: 539–540

    Google Scholar 

  14. Madeley CR, Cosgrove BP (1975) 28 nm particles in infantile gastroenteritis. Lancet ii: 451–452

    Google Scholar 

  15. Moeremans M, Daneels G, Van Dijck A, Langanger G, De Mey J (1984) Sensitive visualisation of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. J Immunol Methods 74: 353–360

    Google Scholar 

  16. Rousset M (1986) The human colon carcinoma cell lines HT-29 and CaCo-2: two in vitro models for the study of intestinal differentiation. Biochemie 68: 1035–1040

    Google Scholar 

  17. Samson ACR, Willcocks MM, Routledge EG, Morgan LA, Toms GL (1986) A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein. J Gen Virol 67: 1479–1483

    Google Scholar 

  18. Snodgrass DR, Gray EW (1977) Detection and transmission of 30 nm virus particles (astrovirus) in faeces of lambs with diarrhoea. Arch Virol 55: 287–291

    Google Scholar 

  19. Willcocks MM, Carter MJ, Laidler FR, Madeley CR (1988) Restriction enzyme characterization of faecal adenoviruses in Newcastle upon Tyne. Epidemiol Infect 101: 445–458

    Google Scholar 

  20. Woode GN, Bridger JC (1978) Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol 11: 441–452

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Willcocks, M.M., Carter, M.J., Laidler, F.R. et al. Growth and characterisation of human faecal astrovirus in a continuous cell line. Archives of Virology 113, 73–81 (1990). https://doi.org/10.1007/BF01318354

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01318354

Keywords

Navigation