Abstract
An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1–10 μm and α-naphthaleneacetic acid (0–0.5 μm showed axillary bud proliferation. Shoots proliferated in vitro were multiplied on Murashige and Skoog medium containing 2.5 μm benzyl adenine and 0.25 μm α-naphthaleneacetic acid. Seasonal changes affected the shoot proliferation potential of the initial explant. Shoots were rooted on a half-strength, growth-regulator-free, agar-gelled, MS medium after a 48-h treatment on half-strength MS liquid medium with 10 μm indole-3-butyric acid. Rooted plantlets were potted and acclimatized in a growth chamber and then moved to the greenhouse. Four-month-old plants were transplanted to the field.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BA :
-
Benzyl adenine
- IBA :
-
Indole-3-butyric acid
- 2-ip :
-
N6-(γ-dimethylallylamino)purine
- MS :
-
Murashige and Skoog (1962) medium
- NAA :
-
α-Naphthaleneacetic acid
References
Gaspar T, Coumans M (1987) Root formation. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol 2. Nijhoff, The Hague, pp 202–221
Hu CY, Wang PJ (1983) Meristem, shoot tip and bud culture. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 1. Macmillan, New York, pp 177–227
Kotwal MM, Gupta PK, Mascarenhas AF (1983) Rapid multiplication ofSapium sebiferum Roxb. by tissue culture. Plant Cell Tissue Organ Cult 2:133–139
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Singh K, Kapur SK, Sarin YK (1993) Domestication ofSapium sebiferum under Jammu conditions. Indian For 16:36–42
Siril EA, Dhar U (1996) A highly efficient in vitro regeneration methodology for mature Chinese Tallow Tree (Sapium sebiferum Roxb.). Plant Cell Rep 16:83–87
Snedecor GW, Cochran WG (1968) Statistical methods. Oxford & IBH, New Delhi
Sujatha M, Dhingra M (1993) Rapid plant regeneration from various explants of Jatropha integerrima. Plant Cell Tissue Organ Cult 35:293–296
Thorpe TA, Biondi S (1984) Conifers. In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2. Macmillan, New York, pp 435–470
Tisserat B (1985) Embryogenesis, organogenesis and plant regeneration. In: Dixon RA (ed) Plant cell culture — A practical approach. IRL, Oxford, pp 79–105
Venketeswaran S, Gandhi V (1982) Mass propagation and genetic improvement of forest trees for biomass production by tissue culture. Biomass 2:5–15
Venketeswaran S (1989) Chinese Tallow (Sapium sebiferum Roxb.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 5, trees II. Springer, Berlin Heidelberg New York, pp 342–358
Zimmerman RH (1991) Micropropagation of temperate zone fruit and nut crops. In: Debergh PC, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer, Dordrecht, pp 231–246
Author information
Authors and Affiliations
Additional information
Communicated by W. Parrott
Rights and permissions
About this article
Cite this article
Siril, E.A., Dhar, U. Micropropagation of mature Chinese tallow tree (Sapium sebiferum Roxb.). Plant Cell Reports 16, 637–640 (1997). https://doi.org/10.1007/BF01275506
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01275506