Abstract
Tree shrews (Tupaia belangeri chinenesis) can be experimentally infected with human hepatitis B virus (HBV) by inoculation with human serum positive for HBV, the experimental infection rate being 55.21%. Successive infections have been passed through five generations among the tree shrews inoculated with HBV-positive sera from the infected animals, the average infection rate being 94.0%. The experimental infection of tree shrews with HBV may be prevented by immunization with hepatitis B vaccine, the protection rate being 88.89%. Standard serum containing HBV at 108 CID (chimpanzee infection dose)/ml, was diluted 10−6, 10−7, 10−8, 10−9, and 10−10 and produced infection rates of 80.0%, 88.8%, 66.7%, 55.6% and 42.9% respectively. Thus the CID50 in tree shrews may reach a dilution of 10−9, which shows that tree shrews are sensitive to HBV infection. These results successfully establish tree shrews as a reliable and useful animal model for research on HBV infection and its relation to hepatocarcinogenesis.
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Abbreviations
- HCC :
-
Hepatocellular carcinoma
- HBV :
-
human hepatitis B virus
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Yan, R.Q., Su, J.J., Huang, D.R. et al. Human hepatitis B virus and hepatocellular carcinoma I. Experimental infection of tree shrews with hepatitis B virus. J Cancer Res Clin Oncol 122, 283–288 (1996). https://doi.org/10.1007/BF01261404
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DOI: https://doi.org/10.1007/BF01261404