The Histochemical Journal

, Volume 14, Issue 5, pp 791–801

The preparation of protein A-gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra-thin sections

  • Jurgen Roth

DOI: 10.1007/BF01033628

Cite this article as:
Roth, J. Histochem J (1982) 14: 791. doi:10.1007/BF01033628


The preparation of a protein A-gold complex (pAg3) using 3 nm gold particles and its application for labelling of intracellular antigens on thin sections is reported. The 3 nm gold particle is the smallest metal particle currently available for cytochemistry and permits a higher resolution of the pAg technique. Furthermore, it can be used in double labelling experiments in conjunction with a pAg complex prepared from 15 nm gold particles. For double labelling, the pAg3 complex must be used for staining of the first antigen since otherwise a non-specific co-labelling of the two pAg complexes results.

Copyright information

© Chapman and Hall Ltd. 1982

Authors and Affiliations

  • Jurgen Roth
    • 1
  1. 1.Institute of Histology and EmbryologyUniversity of Geneva Medical SchoolGeneva 4Switzerland