Research Articles

Molecular Biology Reports

, Volume 22, Issue 1, pp 47-52

Interaction of Co, Mn, Mg and Al with d(GCGTACGC): a spectroscopic study

  • M. T. RajanAffiliated withDepartment of Biochemistry and Nutrition, Central Food Technological Research Institute
  • , D. ChampionAffiliated withDepartment of Physics, Indian Institute of ScienceThe Jawaharlal Nehru Center for Advanced Scientific Research
  • , C. S. KumarAffiliated withDepartment of Physics, Indian Institute of ScienceThe Jawaharlal Nehru Center for Advanced Scientific Research
  • , D. VishnuvardhanAffiliated withDepartment of Biochemistry and Nutrition, Central Food Technological Research Institute
  • , K. S. Jagannatha RaoAffiliated withDepartment of Biochemistry and Nutrition, Central Food Technological Research Institute
  • , M. A. ViswamitraAffiliated withDepartment of Physics, Indian Institute of ScienceThe Jawaharlal Nehru Center for Advanced Scientific Research

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Abstract

Spectroscopic study on the interactions of trace elements Co, Mn, Mg and Al with d(GCGTACGC) indicated the following: Al and Mg did not alter Tm values. Mn enhanced Tm at lower concentration and decreased it at higher concentrations. Interestingly Co at higher concentration elevated the Tm. These studies also showed lower concentrations of Mn displaced EtBr, whereas Al could displace it at higher ionic strength. Mg and Co displaced EtBr fluorescence at moderate concentrations. The binding constant values and CD spectra clearly indicated strong binding of these elements to DNA.

Key words

DNA interaction spectroscopy and trace elements