Neurochemical Research

, Volume 12, Issue 2, pp 173–178

In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine

Authors

  • B. A. Bailey
    • Psychiatric Research Division, Saskatchewan Health, Cancer and Medical Research BuildingUniversity of Saskatchewan
  • S. R. Philips
    • Psychiatric Research Division, Saskatchewan Health, Cancer and Medical Research BuildingUniversity of Saskatchewan
  • A. A. Boulton
    • Psychiatric Research Division, Saskatchewan Health, Cancer and Medical Research BuildingUniversity of Saskatchewan
Original Articles

DOI: 10.1007/BF00979534

Cite this article as:
Bailey, B.A., Philips, S.R. & Boulton, A.A. Neurochem Res (1987) 12: 173. doi:10.1007/BF00979534

Abstract

The in vivo release of endogenous 3,4-dihydroxyphenylethylamine (DA) and its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 3-methoxytyramine (3-MT), and of 5-hydroxytryptamine (5-HT) and its metabolite, 5-hydroxyindoleacetic acid (5-HIAA), has been measured in the caudate nucleus of the anesthetized rat. A push-pull cannula was implanted into the brain, and the tissue perfused with artificial CSF or artificial CSF containing 5×10−4 M phenylethylamine. The perfusate was collected and analyzed for DA, 5-HT and their metabolites by high performance liquid chromatography with electrochemical detection (HPLC-ECD). DA was released by phenylethylamine at rates significantly greater than its basal rate. 3-MT and 5-HT were undetectable in perfusates collected under basal conditions, but could be detected readlly during phenylethylamine stimulation. DOPAC, HVA and 5-HIAA concentrations were not significantly affected by phenylethylamine. The results suggest (1) that phenylethylamine may exert its behavioural effects through increased release of both DA and 5-HT, and (2) that in vivo measurements of the acid metabolites alone may not be indicative of the release of the amines.

Key Words

Dopamine release 5-HT release phenylethylamine push-pull perfusion

Copyright information

© Plenum Publishing Corporation 1987