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In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine

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Abstract

The in vivo release of endogenous 3,4-dihydroxyphenylethylamine (DA) and its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 3-methoxytyramine (3-MT), and of 5-hydroxytryptamine (5-HT) and its metabolite, 5-hydroxyindoleacetic acid (5-HIAA), has been measured in the caudate nucleus of the anesthetized rat. A push-pull cannula was implanted into the brain, and the tissue perfused with artificial CSF or artificial CSF containing 5×10−4 M phenylethylamine. The perfusate was collected and analyzed for DA, 5-HT and their metabolites by high performance liquid chromatography with electrochemical detection (HPLC-ECD). DA was released by phenylethylamine at rates significantly greater than its basal rate. 3-MT and 5-HT were undetectable in perfusates collected under basal conditions, but could be detected readlly during phenylethylamine stimulation. DOPAC, HVA and 5-HIAA concentrations were not significantly affected by phenylethylamine. The results suggest (1) that phenylethylamine may exert its behavioural effects through increased release of both DA and 5-HT, and (2) that in vivo measurements of the acid metabolites alone may not be indicative of the release of the amines.

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Author notes

  1. B. A. Bailey

    Present address: Bio-Research Laboratories, 87 Senneville Road, H9X 3R3, Senneville, Quebec, Canada

Authors and Affiliations

  1. Psychiatric Research Division, Saskatchewan Health, Cancer and Medical Research Building, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

    B. A. Bailey, S. R. Philips & A. A. Boulton

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  1. B. A. Bailey
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  2. S. R. Philips
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  3. A. A. Boulton
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Special Issue Dedicated to Dr. Abel Lajtha.

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Bailey, B.A., Philips, S.R. & Boulton, A.A. In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine. Neurochem Res 12, 173–178 (1987). https://doi.org/10.1007/BF00979534

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