In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The in vivo release of endogenous 3,4-dihydroxyphenylethylamine (DA) and its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 3-methoxytyramine (3-MT), and of 5-hydroxytryptamine (5-HT) and its metabolite, 5-hydroxyindoleacetic acid (5-HIAA), has been measured in the caudate nucleus of the anesthetized rat. A push-pull cannula was implanted into the brain, and the tissue perfused with artificial CSF or artificial CSF containing 5×10−4 M phenylethylamine. The perfusate was collected and analyzed for DA, 5-HT and their metabolites by high performance liquid chromatography with electrochemical detection (HPLC-ECD). DA was released by phenylethylamine at rates significantly greater than its basal rate. 3-MT and 5-HT were undetectable in perfusates collected under basal conditions, but could be detected readlly during phenylethylamine stimulation. DOPAC, HVA and 5-HIAA concentrations were not significantly affected by phenylethylamine. The results suggest (1) that phenylethylamine may exert its behavioural effects through increased release of both DA and 5-HT, and (2) that in vivo measurements of the acid metabolites alone may not be indicative of the release of the amines.
- Durden, D. A., Philips, S. R., and Boulton, A. A. 1973. Identification and distribution of β-phenylethylamine in the rat. Can. J. Biochem. 51:995–1002.
- Saavedra, J. M. 1974. Enzymatic isotopic assay for and presence of β-phenylethylamine in brain. J. Neurochem. 22:211–216.
- Philips, S. R., Rozdilsky, B., and Boulton, A. A. 1978. Evidence for the presence ofm-tyramine,p-tyramine, tryptamine and phenylethylamine in the rat brain and several areas of the human brain. Biol. Psychiat. 13:51–57.
- Reynolds, G. P., Riederer, P., and Sandler, M. 1979. 2-Phenylethylamine and amphetamine in human brain: Effects ofl-deprenyl in Parkinson's disease. Biochem. Soc. Trans. 7:143–145.
- Mantegazza, P., and Riva, M. 1963. Amphetamine-like activity of β-phenylethylamine after a monoamine oxidase inhibitor in vivo. J. Pharm. Pharmacol. 15:472–478.
- Randrup, A., and Munkvad, I. 1966. Dopa and other naturally occurring substances as causes of stereotypy and rage in rats. Acta Psychiat. Scand. 191:193–199.
- Braestrup, C., and Randrup, A. 1978. Stereotyped behavior in rats induced by phenylethylamine, dependence on dopamine and noradrenaline, and possible relation to psychoses. Pages 245–269, in Mosnaim, A. D. and Wolf, M. E. (eds.), Noncatecholic Phenylethylamines, Part 1. Phenylethylamine: Biological Mechanisms and Clinical Aspects, Marcel Dekker, New York.
- Moja, E. A., Stoff, D. M., Gillin, J. C., and Wyatt, R. J. 1976. Dose-response effects of β-phenylethylamine on stereotyped behavior in pargyline-pretreated rats. Biol. Psychiat. 11:731–742.
- Baker, G. B., Raiteri, M., Bertollini, A., Del Carmine, R., Keane, P. E., and Martin, I. L. 1976. Interaction of β-phenylethylamine with dopamine and noradrenaline in the central nervous system of the rat. J. Pharm. Pharmacol. 28:456–457.
- Fuxe, K., Grobecker, H., and Jonsson, J. 1967. The effect of β-phenylethylamine on central and peripheral monoamine containing neurons. Eur. J. Pharmacol. 2:202–207.
- Perlow, M. J., Chiueh, C. C., Lake, C. R., and Wyatt, R. J. 1980. Increased dopamine and norepinephrine concentrations in primate CSF following amphetamine and phenylethylamine administration. Brain Res. 186:469–473.
- Jacobs, B. L. 1976. Minireview: an animal behavior model for studying central serotonergic synapses. Life Sci. 19:777–786.
- Sloviter, R. S., Connor, J. D., and Drust, E. G. 1980. Serotonergic properties of β-phenylethylamine in rats. Neuropharmacology 19:1071–1076.
- Dourish, C. T. 1981. Behavioural effects of acute and chronic β-phenylethylamine administration in the rat: evidence for the involvement of 5-hydroxytryptamine. Neuropharmacology 20:1067–1072.
- Philips, S. R., and Robson, A. M., 1983. In vivo release of endogenous dopamine from rat caudate nucleus by phenylethylamine. Neuropharmacology 22:1297–1301.
- Zetterstrom, T., and Ungerstedt, U. 1984. Effects of apomorphine on the in vivo release of dopamine and its metabolites, studied by brain dialysis. Eur. J. Pharmacol. 97:29–36.
- Zetterstrom, T., Sharp, T., and Ungerstedt, U. 1985. Effect of neuroleptic drugs on striatal dopamine release and metabolism in the awake rat studied by intracerebral dialysis. Eur. J. Pharmacol. 106:27–37.
- Nielsen, J. A., and Moore, K. E. 1982. Measurement of metabolites of dopamine and 5-hydroxytryptamine in cerebroventricular perfusates of unanesthetized, freely moving rats: selective effects of drugs. Pharmacol. Biochem. Behav. 16:131–137.
- Loullis, C. C., Hingtgen, J. N., Shea, P. A., and Aprison, M. H. 1980. In vivo determination of endogenous biogenic amines in rat brain using HPLC and push-pull cannula. Pharmacol. Biochem. Behav. 12:959–963.
- Chen, J.-C., Rhee, K. K., Beaudry, D. M., and Ramirez, V. D. 1984. In vivo output of dopamine and metabolites from the rat caudate nucleus as estimated with push-pull perfusion on-line with HPLC-EC in unrestrained, conscious rats. Neuroendocrinology 38:362–370.
- Philips, S. R., Robson, A. M., and Boulton, A. A. 1982. Unstimulated and amphetamine-stimulated release of endogenous noradrenaline and dopamine from rat brain in vivo. J. Neurochem. 38:1106–1110.
- Yaksh, T. L., and Yamamura, H. I. 1974. Factors affecting performance of the push-pull cannula in the brain. J. Appl. Physiol. 37:428–434.
- Skinner, J. E. 1971. Neuroscience: A Laboratory Manual, W. B. Saunders, Philadelphia.
- Zar, J. H. 1974. Biostatistical Analysis, Prentice-Hall, Englewood Cliffs, N.J.
- Kehr, W. 1976. 3-Methoxytyramine as an indicator in impulse-induced dopamine release in rat brain in vivo. Naunyn-Schmiedeberg's Arch. Pharmacol. 293:209–215.
- Westerink, B. H. C., and Spaan, S. J. 1982. On the significance of endogenous 3-methoxytyramine for the effects of centrally acting drugs on dopamine release in the rat brain. J. Neurochem. 38:680–686.
- Moore, K. E. 1977. The actions of amphetamine on neurotransmitters: a brief review. Biol. Psychiat. 12:451–462.
- Glowinski, J., Axelrod, J., and Iversen, L. L. 1966. Regional studies of catecholamines in the rat brain. IV. Effects of drugs on the disposition and metabolism of3H-norepinephrine and3H-dopamine. J. Pharmacol. Exp. Ther. 153:30–41.
- Horn, A. S., and Snyder, S. H. 1972. Steric requirements for catecholamine uptake by rat brain synaptosomes: studies with rigid analogues of amphetamine. J. Pharmacol. Exp. Ther. 180:523–530.
- Raiteri, M., Del Carmine, R., Bertollini, A., and Levi, G. 1977. Effect of sympathomimetic amines on the synaptosomal transport of noradrenaline, dopamine and 5-hydroxytryptamine. Eur. J. Pharmacol. 41:133–143.
- Nielsen, J. A., Chapin, D. S., and Moore, K. E. 1983. Differential effects ofd-amphetamine, β-phenylethylamine, cocaine and methylphenidate on the rate of dopamine synthesis in terminals of nigrostriatal and mesolimbic neurons and on the efflux of dopamine metabolites into cerebroventricular perfusates of rats. Life Sci. 33:1899–1907.
- Dyck, L. E. 1984. The behavioural effects of phenelzine and phenylethylamine may be due to amine release. Brain Res. Bull. 12:23–28.
- Dourish, C. T., Greenshaw, A. J., and Boulton, A. A. 1983. Deuterium substitution enhances the effects of β-phenylethylamine on spontaneous motor activity in the rat. Pharmacol. Biochem. Behav. 19:471–475.
- Ewing, A. G., and Wightman, R. M. 1984. Monitoring the stimulated release of dopamine with in vivo voltammetry. II: Clearance of released dopamine from extracellular fluid. J. Neurochem. 43:570–577.
- In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine
Volume 12, Issue 2 , pp 173-178
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- Dopamine release
- 5-HT release
- push-pull perfusion
- Industry Sectors