Original Articles

Neurochemical Research

, Volume 13, Issue 1, pp 83-86

First online:

Decreased calmodulin kinase activity after status epilepticus

  • Jeff BronsteinAffiliated withDepartment of Neuroscience, UCLA School of Medicine
  • , Debora FarberAffiliated withDepartment of Ophthalmology, UCLA School of Medicine
  • , Claude WasterlainAffiliated withDepartment of Neuroscience, UCLA School of MedicineDepartment of Neurology, UCLA School of Medicine

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Status epilepticus was induced in paralyzed, ventilated rats using bicuculline and was maintained for 50 to 120 minutes. Cerebral cortex, hippocampus, and cerebellum were assayed for calmodulin kinase II activity in vitro using [γ-32P]ATP and polyacrylamide gel electrophoresis. Seizures resulted in a 3.2 fold decrease in calmodulin kinase activity in crude synaptic membranes of cortex and in a 8.2 fold decrease in hippocampal membranes. Cytosolic calmodulin kinase activity was slightly increased in rats in status epilepticus but statistical significance was not reached. Status epilepticus did not affect calcium/calmodulin-dependent kinase activity in cerebellar membranes or cytosol. These data suggest that intense firing associated with continuous seizure activity decreases calmodulin kinase activity in cortical and hippocampal synaptic membranes, which may result in altered neuronal excitability.

Key Words

Calcium calmodulin kinase protein phosphoylation epilepsy status epilepticus