Decreased calmodulin kinase activity after status epilepticus
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Status epilepticus was induced in paralyzed, ventilated rats using bicuculline and was maintained for 50 to 120 minutes. Cerebral cortex, hippocampus, and cerebellum were assayed for calmodulin kinase II activity in vitro using [γ-32P]ATP and polyacrylamide gel electrophoresis. Seizures resulted in a 3.2 fold decrease in calmodulin kinase activity in crude synaptic membranes of cortex and in a 8.2 fold decrease in hippocampal membranes. Cytosolic calmodulin kinase activity was slightly increased in rats in status epilepticus but statistical significance was not reached. Status epilepticus did not affect calcium/calmodulin-dependent kinase activity in cerebellar membranes or cytosol. These data suggest that intense firing associated with continuous seizure activity decreases calmodulin kinase activity in cortical and hippocampal synaptic membranes, which may result in altered neuronal excitability.
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- Decreased calmodulin kinase activity after status epilepticus
Volume 13, Issue 1 , pp 83-86
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- calmodulin kinase
- protein phosphoylation
- status epilepticus
- Industry Sectors
- Author Affiliations
- 1. Department of Neuroscience, UCLA School of Medicine, 90024, Los Angeles, CA
- 2. Department of Ophthalmology, UCLA School of Medicine, 90024, Los Angeles, CA
- 3. Department of Neurology, UCLA School of Medicine, 90024, Los Angeles, CA