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Arachidonic acid metabolites: Effects on inflammation of fetal rabbit excisional wounds

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Abstract

Uncovered fetal rabbit excisional wounds do not exhibit any classic signs of healing; wounds covered with an impermeable cover do contract, reepithelialize, and exhibit inflammation. Prostaglandin E2 (PGE2) is elevated in amniotic fluid, acting as an immunosuppressant at the maternal-fetal interface. Full-thickness excisional wounds were made on 25-day gestational age rabbit fetuses. Half the wounds were covered with an impermeable cover. Tissue from covered, uncovered, and nonwounded fetuses was examined 72 h after wounding for arachidonic acid metabolites. Uncovered wounds had significantly (P≤0.05) elevated levels of PGE2, PGE2α, and 12-HETE versus covered wounds and control tissue. Covered wounds had significantly elevated levels of 15-HETE compared to uncovered and control tissue. The elevated PGE2 in uncovered wounds may act as a fetal immunosuppressant; covered wounds (lower PGE2) developed cellular inflammation. Further investigations of these interactions may permit modulation of adult inflammation.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Bowman Gray School of Medicine Medical Center Blvd., 27157-1075, Winston-Salem, North Carolina

    Michael J. Morykwas, Debby A. Jennings, M. Stephen Ledbetter & Louis C. Argenta

  2. Department of Pathology, Bowman Gray School of Medicine Medical Center Blvd., 27157-1075, Winston-Salem, North Carolina

    Wain L. White

  3. Department of Dermatology, University of Michigan, 48108-0528, Ann Arbor, Michigan

    Elizabeth A. Duell

Authors
  1. Michael J. Morykwas
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  2. Elizabeth A. Duell
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  4. M. Stephen Ledbetter
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  5. Wain L. White
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  6. Louis C. Argenta
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Morykwas, M.J., Duell, E.A., Jennings, D.A. et al. Arachidonic acid metabolites: Effects on inflammation of fetal rabbit excisional wounds. Inflammation 16, 251–258 (1992). https://doi.org/10.1007/BF00918814

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