Skip to main content
SpringerLink
Log in

Impaired T-lymphocyte colony formation by cord blood mononuclear cells

  • Original Articles
  • Published:
Journal of Clinical Immunology Aims and scope Submit manuscript

Abstract

When compared to adult mononuclear cells, cord blood mononuclear cells demonstrated significantly decreased T-lymphocyte colony formation (1351±643 vs 592±862,P<0.01). This diminished colony-forming activity did not appear to be associated with impaired responsiveness to the stimulant phytohemagglutinin or with excessive suppressor-cell activity. Irradiation reduced the colony-forming capacity of cord blood mononuclear cells more than it did that of adult mononuclear cells. Depletion of adherent cells reduced cord blood mononuclear-cell colony-forming capacity by 40%, while similar treatment reduced adult colony formation by 10%. Lymphocyte proliferation in liquid culture of cord and adult cells was minimally affected by these procedures. The colony-forming capacity of cord blood could be enhanced by the addition of irradiated adult cells (284±72 vs 752±78,P<0.01). This enhancement was demonstrated to be due to a soluble factor produced by a population of irradiated adult cells depleted of the OKT8+ subpopulation of lymphocytes. These results indicate that the progenitor cells of T-lymphocyte colonies in cord blood have distinct biologic characteristics when compared to colony progenitors present in adult blood. This assay may prove to be useful in our efforts to understand the differentiation of T-cell function in man.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Stiehm ER, Winter HS, Bryson YJ: Cellular (T cell) immunity in the human newborn. Pediatrics (Suppl) 64:814–821, 1979

    Google Scholar 

  2. Wara DW, Barrett DJ: Cell-mediated immunity in the newborn: Clinical aspects. Pediatrics (Suppl) 64:822–828, 1979

    Google Scholar 

  3. Handzel TZ, Levin S, Dolphin Z, Schlesinger M, Hahn T, Altman Y, Schechter B, Shneyour A, Trainin N: Immune competence of newborn lymphocytes. Pediatrics 65:491–496, 1980

    Google Scholar 

  4. Rozenszajn LA, Shoham D, Kalechman I: Clonal proliferation of PHA-stimulated human lymphocytes in soft agar culture. Immunology 29:1041–1055, 1975

    Google Scholar 

  5. Fibach E, Gerassi E, Sachs I: Induction of colony formation in vitro by human lymphocytes. Nature (Lond) 259:127–129, 1976

    Google Scholar 

  6. Claësson MH, Rodger MB, Johnson GR, Whittingham S, Metcalf D: Colony formation by human T lymphocytes in agar medium. Clin Exp Immunol 28:526–534, 1976

    Google Scholar 

  7. Herrod HG, Valenski WR: T lymphocyte colony forming capacity of patients with immunodeficiency diseases: Relationship of colony formation to E rosette formation and lymphocyte proliferation. Clin Exp Immunol 45:562–567, 1981

    Google Scholar 

  8. Foa R, Catovsky D, Lauria F: T-lymphocyte colonies in human cord blood. Exp Hematol 8:1139–1145, 1980

    Google Scholar 

  9. Boyum A: Separation of lymphocytes from blood and bone marrow. Scand J Clin Lab Invest 21 (Suppl 97):77–89, 1968

    Google Scholar 

  10. Herrod HG, Buckley RH: Use of a human plaque-forming cell assay to study peripheral blood bursa-equivalent cell activation and excessive suppressor cell activity in humoral immunodeficiency. J Clin Invest 63:863–876, 1979

    Google Scholar 

  11. Hoffman RA, Kung PC, Hansen WP, Goldstein G: Simple and rapid determination of human T lymphocytes and their subclasses in peripheral blood. Proc Natl Acad Sci USA 77:4914–4917, 1980

    Google Scholar 

  12. Reinherz EL, Kung PK, Goldstein G, Levey RH, Schlossman SF: Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T lineage. Proc Natl Acad Sci USA 77:1588–1592, 1980

    Google Scholar 

  13. Jondal M, Wigzell H, Aiuti F: Human lymphocyte subpopulations: Classification according to surface marker and/or functional characterization. Transplant Rev 16:163–195, 1973

    Google Scholar 

  14. Iscove NN, Schreier MH: Clonal growth of cells in semisolid or viscous medium.In Immunological Methods, I Lefkovits, B Pernis (eds). New York, Academic Press, 1979, pp 379–385

    Google Scholar 

  15. Sutherland CC, Dalton C, Wilson JD: T-lymphocyte colonies in malignant disease. Lancet 2:1113–1115, 1976

    Google Scholar 

  16. Whittingham S, Rodger B, Matthews JV, MacKay IR: Cloning of T lymphocytes in systemic lupus erythematosis. Clin Exp Immunol 34:63–68, 1978.

    Google Scholar 

  17. Claësson MH, Andersen V: Impaired T lymphocyte colony formation in infectious mononucleosis: Evidence for both monocyte and lymphocyte defects. Clin Exp Immunol 38:483–491, 1979

    Google Scholar 

  18. Stahn R, Falricius HA, Hartleitner W: Suppression of human T-cell colony formation during pregnancy. Nature 276:831–832, 1978

    Google Scholar 

  19. Olding LB, Oldstone MBA: Lymphocytes from human newborns abrogate mitosis of their mothers' lymphocytes. Nature 249:161–162, 1974

    Google Scholar 

  20. Hayward AR, Lawton AR: Induction of plasma cell differentiation of human fetal lymphocytes: Evidence for functional immaturity of T and B cells. J Immunol 119:1213–1217, 1977

    Google Scholar 

  21. Morito T, Bankhurst AD, Williams RC: Studies of human cord blood and adult lymphocyte interactions within vitro immunoglobulin production. J Clin Invest 64:990–995, 1979

    Google Scholar 

  22. Tosato G, Magrath IT, Koski IR, Dooley J, Blaese RM: B cell differentiation and immunoregulatory T cell function in human cord blood lymphocytes. J Clin Invest 66:383–388, 1980

    Google Scholar 

  23. Janossy G, Tidman N, Papageorgiou ES, Kung PC, Goldstein G: Distribution of T lymphocyte subsets in the human bone marrow and thymus. An analysis with monoclonal antibodies. J Immunol 126:1608–1613, 1981

    Google Scholar 

  24. Rodriguez MA, Bankhurst AD, Ceuppers JL, William RC: Characterization of the suppressor cell activity in human cord blood lymphocytes. J Clin Invest 68:1577–1585, 1981

    Google Scholar 

  25. Gelfand EW, Lee JWW, Dosch H-M, Price GG: Human T cell colony formation in microculture: Analysis of growth requirements and functional activities. J Immunol 126:1134–1139, 1981

    Google Scholar 

  26. Rosenstreich DL, Wilton JM: The mechanism of action of macrophages in activation of T-lymphocytesin vitro by antigens and mitogens.In Proceedings, Ninth Leukocyte Culture Conference, AS Rosenthal (ed). New York, Academic Press, 1975, pp 113–132

    Google Scholar 

  27. Hansen GS, Rubin G, Sorensen SF: Human leukocyte responsesin vitro. Transformation of purified T-lymphocytes with and without addition of partially purified monocytes. Clin Exp Immunol 29:295–303, 1977

    Google Scholar 

  28. Unanue ER: The regulation of lymphocyte function by themacrophage. Immunol Rev 40:227–255, 1978

    Google Scholar 

  29. Gerassi E, Sachs L: Regulation of human T cell colonies by an inducing activity (TCI) produced by normal human and malignant mouse cells. J Immunol 121:2547–2551, 1978

    Google Scholar 

  30. Mercola K, Cline MJ: A new clonogenic technique for human mitogen responsive T cells. J Immunol 123:1721–1725, 1979

    Google Scholar 

  31. Spitzer G, Verma DS, Zander A, Beran M, Diche KA, Siegel S: One step procedure for human T-lymphocyte colony growth and its regulation by T and B cells and monocytes. Exp Hematol 10:1241–1252, 1980

    Google Scholar 

  32. Triebal F, Robinson WA, Hayward AR, Goube de La Forest P: Characterization of the T lymphocyte colony forming cells and evidence of the acquisition of T cell markers in the absence of the thymic microenvironment in man. J Immunol 126:2020–2023, 1981

    Google Scholar 

  33. Hayward AR, Kurnich J: Newborn T cell suppression: Early appearance, maintenance in culture and lack of growth suppression. J Immunol 126:50–53, 1981

    Google Scholar 

  34. Douer D, Sachs L: Production of human T cell colony inducing activity (TCI) by malignant cells from patients with lymphocytic and myeloid leukemia. J Immunol 122:2473–2477, 1979

    Google Scholar 

  35. Vesole DH, Moore GE: The induction of human peripheral blood lymphoid colonies by conditioned media from human tumor lines. Immunology 41:723–728, 1980

    Google Scholar 

  36. Hayward AR, Lydyard PM: B cell function in the newborn. Pediatrics 64:758–763, 1979

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, University of Tennessee Center for the Health Sciences, 38163, Memphis, Tennessee

    Henry G. Herrod & William R. Valenski

Authors
  1. Henry G. Herrod
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William R. Valenski
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Herrod, H.G., Valenski, W.R. Impaired T-lymphocyte colony formation by cord blood mononuclear cells. J Clin Immunol 2, 319–326 (1982). https://doi.org/10.1007/BF00915074

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00915074

Key words

Search

Navigation