Summary
Pairs of eye-antennal discs, attached to the cephalic ganglia, were cultured in vitro with a concentration of β-ecdysone optimal for imaginal differentiation. The eye-antennal discs fused to form a vesicle inside which the antennae were partially everted, and on the inner surface of which imaginal structures differentiated. The epithelium of the discs was continuous, and an integrated pattern of bristles and hairs differentiated in vitro. In particular, the median ocellus, a unified structure derived partially from each disc, differentiated normally.
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References
Birmingham, L.: Boundaries of differentiation of cephalic imaginal discs inDrosophila. J. Exp. Zool.91, 345–363 (1942)
Bryant, P.J.: Pattern formation in imaginal discs. In: Genetics and Biology ofDrosophila (in press) (T.R.F. Wright and M. Ashburner, eds.), London: Academic Press 1978
Bullmore, D.: The differential action of α- and β-ecdysone on the division of imaginal disc cells ofDrosophila melanogaster in vitro. Ph.D. Thesis, University of Fribourg 1977
Demal, J., Leloup, A.M.: Organ culture of insects. in: Invertebrate Tissue Culture (C. Vago ed.) Vol. 2, pp. 3–39. London, New York: Academic Press 1972
Edwards, J.S., Milner, M.J., Chen, S.W.: Integument and sensory nerve differentiation ofDrosophila imaginal discs in vitro. Wilhelm Roux's Archives185, 59–77 (1978)
Haynie, J.L.: Intercalery regeneration and pattern formation in the imaginal discs ofDrosophila melanogaster. Ph.D. Thesis, University of California, Irvine 1975
Milner, M.J.: The eversion and differentiation ofDrosophila melanogaster leg and wing imaginal discs cultured in vitro with an optimal concentration of β-ecdysone. J. Embryol. Exp. Morphol.37, 105–117 (1977)
Milner, M.J., Sang, J.H.: Relative activities of α-ecdysone and β-ecdysone for the differentiation in vitro ofDrosophila melanogaster imaginal discs. Cell3, 141–143 (1974)
Russell, G.B., Fenemore, P.G., Horn, D.H.S., Middleton, E.J.: Insect moulting hormones: the phytoecdysones ofDacrydium intermedium. Aust. J. Chem.,25, 1935–1941 (1972)
Schaerer, H.: The action of Mitomycin C on the bristle-forming apparatus ofPhormia regina. Wilhelm Roux's Archives179, 145–158 (1976)
Schneider, I.: Differentiation of larvalDrosophila eye-antennal discs in vitro. J. Exp. Zool.156, 91–104 (1964)
Schneider, I.: Histology of larval eye-antennal disks and cephalic ganglia ofDrosophila cultured in vitro. J. Embryol. Exp. Morphol.15, 271–279 (1966)
Shields, G., Sang, J.H.: Improved medium for culture ofDrosophila embryonic cells. Dros. Inform. Serv.52, 161 (1977)
Snodgrass, R.E.: Principles of Insect Morphology, New York: McGraw-Hill 1935
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Milner, M.J., Haynie, J.L. Fusion ofDrosophila eye-antennal imaginal discs during differentiation in vitro. Wilhelm Roux' Archiv 185, 363–370 (1979). https://doi.org/10.1007/BF00848522
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DOI: https://doi.org/10.1007/BF00848522