High-density culture of FM-3A cells using a bioreactor with an external tangential-flow filtration device
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A novel bioreactor system developed for high-density cultures of suspended mammalian cells is described using a tangential-flow filtration device outside the culture vessel to separate viable cells from spent medium. The filtration device is based on thin porous microfiltration membranes with a pore size of 0.20–0.65 μm. Because cells have a diameter of about 10–20 μm, they cannot permeate these membranes with the spent medium. So, allowing a perfusion culture to be created using this system. In most membrane filtration systems, clogging of the membranes has made long-term operation difficult. In this system, however, high pressure is not applied directly to the membrane, thus minimizing clogging. Also, clogging of the membrane was prevented by washing the membrane surface once a day, and increasing the membrane surface are. With this system, FM-3A cells were cultured and maintained at a high density of 3.0×107 cells/ml for two weeks, and a continuous culture was supported for as long as 34 days.
- Broise D, Noiseux M, Massie B and Lemieux R (1992) Hybridoma perfusion systems: A comparison study. Biotech. Bioeng. 40: 25–32.
- Fraune E (1989) Filtration systems for the perfusion of cell culture media. Paper presented at the 1st Taunus Conference Membrane Technology, 3/89 Bad Soden, West-Germany.
- Himmelfarb P, Thayer PS and Martin HE (1969) Spin filter culture: The propagation of cells in suspension. Science 164: 555–557.
- Knazek PA, Gullino PM, Kohler PO and Dedrick RL (1972) Cell culture on artificial capillaries. Science 178: 65–67.
- Koyama H (1980) FM-3A cell line. Soshiki Baiyou 6(7): 235–243.
- Lim F and Moss RD (1981) Microencapsulation of living cells and tissues. J. Pharm. Sci. 70: 351–354.
- Nilsson K, Scheirer W, Merten OW, Osteberg L, Liehl E, Katinger HWD and Mosbach K (1983) Entrapment of animal cells for production of monoclonal antibodies and other biochemicals. Nature 302: 629–630.
- Satoh S, Kawamura K and Fujiyoshi N (1983) Animal cell cultivation of biological substances with a novel perfusion culture apparatus. J. Tissue Culture Methods 8: 167–171.
- Seamans CT and Hu W-S (1990) Kinetics of growth and antibody production by hybridoma cell line in a perfusion culture. J. Ferm. Bioeng. 70: 241–245.
- Tokashiki M, Hamamoto K, Takazawa Y and Ichikawa Y (1988) High-density culture of mouse-human hybridoma cells using a new perfusion culture vessel. Kagaku Kogaku Ronbunshu 14: 337–341.
- Tokashiki M, Arai T, Hamamoto K and Ishimaru K (1990) High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge. Cytotechnology 3: 239–244.
- Velez D, Miller L and Macmillan DJ (1989) Use of tangential flow filtration in perfusion propagation of hybridoma cells for production of monoclonal antibodies. Biotech. Bioeng. 33: 938–940.
- High-density culture of FM-3A cells using a bioreactor with an external tangential-flow filtration device
Volume 14, Issue 1 , pp 61-66
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- Kluwer Academic Publishers
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- FM-3A cells
- high-density culture
- perfusion culture
- tangential-flow filtration
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