Original Papers

Archives of Microbiology

, Volume 139, Issue 1, pp 44-47

Co-induction of sucrose transport and invertase activities in a thermophilic fungus,Thermomyces lanuginosus

  • P. PalaniveluAffiliated withDepartment of Biochemistry, Indian Institute of Science
  • , P. V. BalasubramanyamAffiliated withDepartment of Biochemistry, Indian Institute of Science
  • , Ramesh MaheshwariAffiliated withDepartment of Biochemistry, Indian Institute of Science

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Abstract

The incorporation of sucrose into the thermophilic fungus,Thermomyces lanuginosus, occurred only in mycelia previously exposed to sucrose or raffinose. Sucrose uptake and invertase were inducible. Both activities appeared in sucrose-induced mycelia at about the same time. Both activities declined almost simultaneously following the exhaustion of sucrose in the medium. The sucrose-induced uptake system was specific for β-fructofuranosides as revealed by competition with various sugars. The induction of sucrose uptake system was blocked by cycloheximide, showing that it was dependent on new protein synthesis. Transport of sucrose did not seem to be dependent on ATP. Rather, uptake of this sugar seemed to be driven by a proton gradient across the plasma membrane. The uptake system showed Michaelis-Menten kinetics.

Key words

Sucrose uptake Transport Invertase Thermomyces lanuginosus Thermophilic fungus