Archives of Microbiology

, Volume 139, Issue 1, pp 44–47

Co-induction of sucrose transport and invertase activities in a thermophilic fungus,Thermomyces lanuginosus

Authors

  • P. Palanivelu
    • Department of BiochemistryIndian Institute of Science
  • P. V. Balasubramanyam
    • Department of BiochemistryIndian Institute of Science
  • Ramesh Maheshwari
    • Department of BiochemistryIndian Institute of Science
Original Papers

DOI: 10.1007/BF00692710

Cite this article as:
Palanivelu, P., Balasubramanyam, P.V. & Maheshwari, R. Arch. Microbiol. (1984) 139: 44. doi:10.1007/BF00692710

Abstract

The incorporation of sucrose into the thermophilic fungus,Thermomyces lanuginosus, occurred only in mycelia previously exposed to sucrose or raffinose. Sucrose uptake and invertase were inducible. Both activities appeared in sucrose-induced mycelia at about the same time. Both activities declined almost simultaneously following the exhaustion of sucrose in the medium. The sucrose-induced uptake system was specific for β-fructofuranosides as revealed by competition with various sugars. The induction of sucrose uptake system was blocked by cycloheximide, showing that it was dependent on new protein synthesis. Transport of sucrose did not seem to be dependent on ATP. Rather, uptake of this sugar seemed to be driven by a proton gradient across the plasma membrane. The uptake system showed Michaelis-Menten kinetics.

Key words

Sucrose uptake Transport Invertase Thermomyces lanuginosus Thermophilic fungus

Abbreviations

FCCP

carbonylcyanide p-trifluoromethylphenyl hydrazone

2,4-DNP

2,4-dinitrophenol

Copyright information

© Springer-Verlag 1984