Abstract
A methanogenic bacterial consortium was obtained after inoculation of benzoate medium under N2/CO2 atmosphere with intertidal sediment. A hydrogen donating organotroph andMethanococcus mazei were isolated from this enrichment. H2-utilising sulphate reducing bacteria were isolated under H2/CO2 in the absence of organic electron donors. TheMethanococcus was able to produce methane in yeast extract medium under N2/CO2 if the H2 donating organism was present, and sulphate reduction occurred if the hydrogen utilising sulphate reducing bacteria were grown with the H2 donating organism. The ability of the H2 utilising sulphate reducing bacteria to inhibitMethanococcus competitively was shown in cultures containing both of these H2 utilising bacteria.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- HDO:
-
hydrogen donating organism
- SRB:
-
sulphate reducing bacteria
- HSRB:
-
hydrogen utilising sulphate reducing bacteria
References
Bell, G. R., LeGall, J., Peck, H. D.: Evidence for the periplasmic location of hydrogenase inDesulfovibrio gigas. J. Bacteriol.120, 994–997 (1974)
Bryant, M. P., Campbell, L. L., Ready, C. A., Crabill, M. R.: Growth ofDesulfovibrio in lactate or ethanol media low in sulfate in association with H2-utilizing methanogenic bacteria. Appl. Env. Microbiol.33, 1162–1169 (1977)
Bryant, M. P., Wolin, M. J.: Rumen bacteria and their metabolic interaction. Proceedings of the first intersectional congress of the International Assoc. Soc., Vol. 2, Developmental Microbiology, Ecology, pp. 297–306. Tokyo: Science Council of Japan 1975
Bryant, M. P., Wolin, E. A., Wolin, M. J., Wolfem, R. S.:Methanobacillus omelianskii, a symbiotic association of two species of bacteria. Arch. Mikrobiol.59, 20–31 (1967)
Buchanan, R. E., Gibbons, N. E. (eds.) Bergey's manual of determinative bacteriology, 8th ed. Baltimore: Williams and Wilkins 1974
Cappenberg, Th. E.: Interrelations between sulfate-reducing and methane producing bacteria in bottom deposits of a freshwater lake. 1. Field observations. Antonie van Leeuwenhoek. J Mikrobiol. Serol.40, 285–295 (1974)
Cappenberg, Th. E.: Relationships between sulfate-reducing and methane producing bacteria. Plant Soil43, 123–139 (1975)
Ferry, J. G., Wolfe, R. S.: Anaerobic degradation of benzoate to methane by a microbial consortium. Arch. Microbiol.107, 33–40 (1976)
Hungate, R. E.: A roll-tube method for cultivation of strict anaerobes. In: Methods in Microbiology (J. R. Norris, D. W. Ribbons, eds.), Vol. 3B, pp. 117–132. New York: Academic Press 1969
Iannotti, E. L., Kafkewitz, D., Wolin, M. J., Bryant, M. P.: Glucose fermentation products ofRuminococcus albus grown in continuous culture, withVibrio succinogenes: Changes caused by interspecies transfer of H2. J. Bacteriol.114, 1231–1240 (1973)
Khosrovi, B., Macpherson, R., Miller, J. D. A.: Some observations of growth and hydrogen uptake byDesulfovibrio vulgaris. Arch. Mikrobiol.80, 324–337 (1971)
Martens, C. S., Berner, R. A.: Methane production in the interstitial waters of sulfate-depleted marine sediments. Science185, 1167–1169 (1974)
Postgate, J. R.: On the nutrition ofDesulphovibrio desulphuricans. A correction. J. Gen Microbiol.9, 440–444 (1953)
Postgate, J. R.: Versatile medium for the enumeration of sulphate reducing bacteria. Appl. Microbiol.11, 265–267 (1963)
Postgate, J. R.: Recent advances in the study of sulfate reducing bacteria. Bact. Rev.29, 425–441 (1965)
Scheifinger, C. C., Lineham, B., Wolin, M. J.: H2 production bySelenomonas ruminantium in the absence and presence of methanogenic bacteria. Appl. Microbiol.29, 480–483 (1975)
Sorokin, Y. I.: Experimental, investigation of bacterial sulfate reduction in the Black Sea. Microbiology32, 320–335 (1962)
Sorokin, Y. I.: Role of carbon dioxide and acetate in biosynthesis by sulphate reducing bacteria. Nature (London)210, 551–552 (1966)
Thauer, R. K., Jungermann, K., Decker, K.: Energy conservation in chemotrophic anaerobic bacteria. Bact. Rev.41, 100–180 (1977)
Thorstenson, D. C.: Equilibrium distribution of small organic molecules in natural waters. Geochim. Cosmochim. Acta34, 745–770 (1970)
Wolin, E. A., Wolin, M. J., Wolfe, R. S.: Formation of methane by bacterial extracts. J. Biol. Chem.238, 2882–2886 (1963)
Winfrey, M. R., Zeikus, J. G.: Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl. Env. Microbiol.33, 275–281 (1977)
Zeikus, J. G., Weimer, P. J., Nelson, D. R., Daniels, L.: Bacterial methanogenesis: Acetate as a methane precursor in pure culture. Arch. Microbiol.104, 129–134 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abram, J.W., Nedwell, D.B. Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Arch. Microbiol. 117, 89–92 (1978). https://doi.org/10.1007/BF00689356
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00689356