Summary
It was the aim of the present experiment to detect possible effects of branched-chain amino acids (BCAA) on the endocrine response to 1 h of continuous running. Blood samples were collected from 14 long-distance runners (age 24–42 years) in two different trials performed at 1-week intervals. In both trials (E and P) blood samples were collected at the following times: 9 a.m. (basal values sample), 10.30 a.m. (sample 90), 11.30 a.m. (sample 150), 12.30 p.m. (sample 210); the athletes performed 1 h of running at a constant predetermined speed between samples 90 and 150. Following the basal sample a mixture containing BCAA (E trial), or not containing BCAA (P trial) was ingested. In both trials no hormone basal concentrations, except insulin, were changed before exercise. In P trial, following exercise (sample 150), human growth hormone (HGH), prolactin (PRL), adrenocorticotropic hormone (ACTH) and cortisol (C) increased, while testosterone (T) decreased. In sample 210, after 1 h of rest, while ACTH, PRL and HGH had recovered to basal concentrations, C remained elevated and T displayed a further decrease. In the E trial a similar pattern of change was observed in sample 150 for HGH, PRL, ACTH and C; in sample 210 HGH and PRL displayed significantly lower values than in the corresponding P trial samples. The T was not modified by the running exercise and increased during the recovery period. It is, therefore, suggested that BCAA administration before exercise affects the response of some anabolic hormones, mainly HGH and T.
References
Arcelli E (1989) La maratona: allenamento e alimentazione. Insport, Milan, pp 243–244
Bargossi AM, Piazzi S, Fiorella PL, Sprovieri C, Zuliani U, Lubich T (1990) Gli aminoacidi nel metabolismo delle attività sportive di potenza. Med Sport 43:115–121
Blomstrand E, Celsing F, Newsholme EA (1988) Changes in plasma concentrations of aromatic and branched-chain amino acids during sustained exercise in men and their possible role in fatigue. Acta Physiol Scand 133:115–121
Bond A, Davis C (1987) Sex hormone binding globulin in clinical perspective. Acta Obstet Gynecol Scand 66:255–262
Buse MG (1981) In vivo effects of branched chain aminoacids on muscle protein synthesis in fasted rats. Horm Metab Res 13:502–505
Carli G, Martelli G, Viti A, Baldi L, Bonifazi M, Lupo di Prisco C (1983) Modulation of hormone levels in male swimmers during training. In: Hollander PA, Huijing PA, deGroot G (eds) Biomechanics and medicine in swimming. Human Kinetics, Champaign, Ill., pp 33–40
Carli G, Bonifazi M, Lodi L, Lupo C, Martelli G, Viti A (1986) Hormonal and metabolic effects following a football match. Int J Sports Med 7:36–38
Carlson HE, Miglietta JT, Roginsky MS, Stegink LD (1989) Stimulation of pituitary hormone secretion by neurotransmitter amino acids in humans. Metabolism 38:1179–1182
Castellino P, Luzi L, Simonson DC, Haymond M, DeFronzo RA (1987) Effect of insulin and plasma amino acid concentrations of leucine metabolism in man. J Clin Invest 80:1784–1793
Chang TW, Goldberg AL (1978) The metabolic fates of amino acids and the formation of glutamine in skeletal muscle. J Biol Chem 253:3685–3693
Conlay LA, Wurtman RJ, Lopez I, Blusztajn JK, Vacanti CA, Logue M, During M, Caballero B, Maher TJ, Evoniuk G (1989) Effect of running the Boston marathon on plasma concentration of large neutral aminoacids. J Neural Transm 76:65–71
Cortesi A, Landi D, Crescenzi F, Santoro F, Gallucci G, Inaudi P (1987) Uso di tracciante iodato per la determinazione dei livelli plasmatici di androstenedione: valutazioni metodologiche e cliniche. Boll Soc Ital Biol Sper 63:81–87
Cumming DC, Quigley ME, Yen SSC (1983) Acute suppression of circulating testosterone level by cortisol in men. J Clin Endocrinol Metab 57:671–673
Dohm GL, Louis TM (1978) Changes in androstenedione, testosterone and protein metabolism as a result of exercise. Proc Soc Exp Biol Med 158:622–625
Fernstrom JD (1983) Role of precursor availability in control of monoamine biosynthesis in brain. Physiol Rev 63:484–546
Fotherby K, Pal SB (1985) Exercise endocrinology. De Gruyter, Berlin
Frick GP, Goodman HM (1989) Insulin regulation of the activity and phosphorylation of branched chain 2 oxo-acid dehydrogenase in adipose tissue. Biochem J 258:229–235
Galbo H (1983) Hormonal and metabolic adaptation to exercise. Thieme, Stuttgart
Garlick PJ, Grant I (1988) Amino acid infusion increases the sensitivity of muscle protein synthesis in vivo to insulin. Biochem J 254:579–584
Heck H, Mader A, Hess G, Mücke S, Müller R, Hollmann W (1985) Justification of the 4 mmol/l lactate threshold. Int J Sports Med 6:117–130
Hutton JC, Sener A, Malaisse WJ (1980) Interaction of branched chain amino acids and keto acids upon pancreatic islet metabolism and insulin secretion. J Biol Chem 255:7340–7346
Isidori A, Lo Monaco A, Cappa M (1981) A study of growth hormone release in man after oral administration of amino acids. Curr Med Res Opin 7:475–481
Jacobson BH (1990) Effect of amino acids on growth hormone release. Phys Sports Med 18:63–70
Keizer H, Janssen GME, Menheere P, Kranenburg G (1989) Changes in basal plasma testosterone, cortisol and dehydroepiandrosterone sulfate in previously untrained males and females preparing for a marathon. Int J Sports Med 10:S139-S145
Kindermann W, Simon G, Keul J (1979) The significance of the aerobic-anaerobic transition for determination of workload intensities during endurance training. Eur J Appl Physiol 42:25–34
Knopf RF, Conn JW, Fajans SS (1965) Plasma growth hormone response to intravenous administration of amino acids. J Clin Endocrinol 25:1140–1144
Koivisto VA, Soman V, Conrad P, Hendler R, Nadel E, Felig P (1979) Insulin binding to monocytes in trained athletes. J Clin Invest 64:1011–1015
Kraemer WJ (1988) Endocrine response to resistance exercise. Med Sci Sports Exerc [Suppl] 20:S152–5157
Kuoppasalmi K, Härkönen M (1985) Steroid hormones in muscular exercise. In: Ljungqvist A, Peltokallio P, Tikkanen H (eds) Sport medicine in track and field athletes. IAF, Kouvola, pp 131–135
Lancranjan IA, Wirz-Justice A, Puhringer W, Del Pozo E (1977) Effect of L-5-hydroxytryptophan infusion of growth hormone and prolactin secretion in man. J Clin Endocrinol Metab 45:588–593
Lupo C, Baldi L, Bonifazi M, Lodi L, Martelli G, Viti A, Carli G (1985) Androgen levels following a football match. Eur J Appl Physiol 54:494–496
Maresh CM, Allison TG, Noble BJ, Drash A, Kraemer WJ (1989) Substrate and hormone responses to exercise following a marathon run. Int J Sports Med 10:101–106
Martelli G, Bonifazi M, Lodi L, Lupo C, Viti A, Carli G (1987) Androgen levels in long distance runners. In: Tsopanakis A, Poortmans J (eds) Physiological biochemistry of exercise and training. Hellenic Sport Research Institute, Athens, pp 215–219
Miller N, Smith AP, Walker AM (1986) Increased dietary leucine changes the rhythm of prolactin secretion. Metabolism 35:622–626
Näveri H, Kuoppasalmi K, Härkönen M (1985) Metabolic and hormonal changes in moderate and intense long-term running exercises. Int J Sports Med 6:276–281
Odessey R, Kairallah EA, Goldberg AL (1974) Significance of alanine production by skeletal muscle. J Biol Chem 249:245–258
Refsum HE, Gjessing LR, Strömme SB (1979) Changes in plasma amino acid distribution and urine amino acids excretion during prolonged heavy exercise. Scand J Clin Lab Invest 39:407–413
Rennie MJ, Edwards RHT, Davies CTM, Krynawich S, Halliday D, Waterlow JC, Milward DJ (1980) Protein and aminoacid turnover during and after exercise. Biochem Soc Trans 8:499–501
Shephard RJ (1982) Physiology and biochemistry of exercise. Praeger, New York
Simmons PS, Miles JM, Gerich JE, Haymond MW (1984) Increased proteolysis. An effect of increases in plasma cortisol within the physiological range. J Clin Invest 73:412–420
Snedecor GW, Cochran WG (1967) Statistical methods. Iowa State University Press, Ames
Snochowski M, Saartik T, Dahlberg E (1981) Androgen and glucocorticoid receptors in human skeletal cytosol. J Steroid Biochem 14:765–771
Vermeulen A, Verdonck L, Van der Straeten M (1969) Capacity of testosterone binding globulin in human plasma and the influence on specific binding of testosterone on its metabolic clearance rate. J Clin Endocrinol Metab 29:1470–1480
Wagenmakers AJM, Brookes JH, Coakley JH, Reilly T, Edwards RHT (1989) Exercise-induced activation of branched-chain 2oxo acid dehydrogenase in human muscle. Eur J Appl Physiol 59:159–167
Wheeler GD, Wall SR, Belcastro AN, Cumming DC (1984) Reduced serum testosterone and prolactin in male distance runners. JAMA 282:514–516
Wingfield JC, Farner DS (1975) The determination of five steroids in avian plasma by radioimmunoassay and competitive protein binding. Steroids 26:311–327
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Carli, G., Bonifazi, M., Lodi, L. et al. Changes in the exercise-induced hormone response to branched chain amino acid administration. Europ. J. Appl. Physiol. 64, 272–277 (1992). https://doi.org/10.1007/BF00626291
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DOI: https://doi.org/10.1007/BF00626291