Skip to main content
Log in

Low- and intermediate-copy-number cloning vectors based on thePseudomonas plasmid pVS1

  • Short Communication
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

The cloning vector pME290 (6.8 kb), which is derived from thePseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1–3 copies) and pME294 (about 15–20 copies) were isolated. These vectors were used in the characterization of theP. aeruginosa argF gene encoding ornithine carbamoyltransferase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bagdasarian M.M., E. Amann, R. Lurz, B. Rückert & M. Bagdasarian (1983) Activity of the hybridtrp-lac(tac) promoter ofEscherichia coli inPseudomonas putida. Construction of broadhost-range, controlled-expression vectors. Gene 26: 273–282

    Google Scholar 

  • De Reuse H., E. Touati, P. Glaser & A. Danchin (1986) Low copy number plasmid vectors for gene cloning and for monitoring gene expression. FEMS Microbiol. Lett. 37: 193–196

    Google Scholar 

  • Haas D., B.W. Holloway, A. Schamböck & T. Leisinger (1977) The genetic organization of arginine biosynthesis inPseudomonas aeruginosa. Mol. Gen. Genet. 154: 7–22

    Google Scholar 

  • Hashimoto-Gotoh T., F.C.H. Franklin, A. Nordheim & K.N. Timmis (1981) Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors. Gene 16: 227–235

    Google Scholar 

  • Humphreys G.O., G.A. Willshaw, H.R. Smith & E.S. Anderson (1976) Mutagenesis of plasmid DNA with hydroxylamine: Isolation of mutants of multi-copy plasmids. Mol. Gen. Genet. 145: 101–108

    Google Scholar 

  • Isaac J. H. & B.W. Holloway (1972) Control of arginine biosynthesis inPseudomonas aeruginosa. J. Gen. Microbiol. 73: 427–438

    Google Scholar 

  • Itoh Y., J.M. Watson, D. Haas & T. Leisinger (1984) Genetic and molecular characterization of thePseudomonas plasmid pVS1. Plasmid 11: 206–220

    Google Scholar 

  • Itoh Y. & D. Haas (1985) Cloning vectors derived from thePseudomonas plasmid pVS1. Gene 36: 27–36

    Google Scholar 

  • Jeenes D.J., L. Soldati, H. Baur, J.M. Watson, A. Mercenier, C. Reimmann, T. Leisinger & D. Haas (1986) Expression of biosynthetic genes fromPseudomonas aeruginosa andEscherichia coli in the heterologous host. Mol. Gen. Genet. 203: 421–429

    Google Scholar 

  • King A., K. Shannon & I. Phillips (1988) In vitro antibacterial activity and susceptibility of cefsulodin, an antipseudomonal cephalosporin, to beta-lactamases. Antimicrob. Agents Chemother. 17: 165–169

    Google Scholar 

  • Leisinger T., D. Haas & M.P. Hegarty (1972) Indospicine as an arginine antagonist inEscherichia coli andPseudomonas aeruginosa. Biochim. Biophys. Acta 262: 214–219

    Google Scholar 

  • Miller R.V. & T.A. Kokjohn (1987) Cloning and characterization of thecI repressor ofPseudomonas aeruginosa bacteriophage D3: a functional analog of phage lambdacI protein. J. Bacteriol. 169: 1847–1852

    Google Scholar 

  • Philippon A., G. Paul & P. Nevot (1981) Comparative in-vitro activity of cefsulodin and ceftazidime against ticarcillin-resistantPseudomonas aeruginosa. J. Antimicrob. Chemother. 8 Suppl. B: 119–122

    Google Scholar 

  • Rella M. & D. Haas (1982) Resistance ofPseudomonas aeruginosa PAO to nalidixic acid and low levels of β-lactam antibiotics: mapping of chromosomal genes. Antimicrob. Agents Chemother. 22: 242–249

    Google Scholar 

  • Som T. & J.-I. Tomizawa (1983) Regulatory regions of ColEl that are involved in determination of plasmid copy number. Proc. Natl. Acad. Sci. USA 80: 3232–3236

    Google Scholar 

  • Stanisich V.A. & B.W. Holloway (1972) A mutant sex factor ofPseudomonas aeruginosa. Genet. Res. 19: 91–108.

    Google Scholar 

  • Voellmy R. & T. Leisinger (1978) Regulation of enzyme synthesis in the arginine biosynthetic pathway ofPseudomonas aeruginosa. J. Gen. Microbiol. 109: 25–35

    Google Scholar 

  • Uhlin B.E. & K. Nordström (1977) R Plasmid gene dosage effects inEscherichia coli K-12: Copy mutants of the R plasmid Rldrd-19. Plasmid 1: 1–7

    Google Scholar 

  • Wang K., S.E. Stachel, B. Timmerman, M. Van Montagu & P.C. Zambryski (1987) Site-specific nick in the T-DNA border sequence as a result ofAgrobacterium vir gene expression. Science 235: 587–591

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Itoh, Y., Soldati, L., Leisinger, T. et al. Low- and intermediate-copy-number cloning vectors based on thePseudomonas plasmid pVS1. Antonie van Leeuwenhoek 54, 567–573 (1988). https://doi.org/10.1007/BF00588392

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00588392

Key words

Navigation