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Morphological changes in strains of Aspergillus flavus link ex fries and Aspergillus parasiticus speare related with aflatoxin production

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Abstract

Two strains ofAspergillus flavus Linkex Fr. and two strains ofA. parasiticus Speare were cultured on crushed moist wheat (Triticum durum var. Pané no. 247) for aflatoxin production studies in correlation with morphological changes. The toxicogenic strains were adapted to the substratum by means of successive transfers at regular intervals (72 h.)

The amount aflatoxins synthesized by the toxicogenic strains decreased gradually after succesive subculturing. The decrease was accompanied by marked morphological changes. One of the strains studied,A. flavus NRRL 3251, lost completly the capacity of aflatoxin synthesis after several subcultures, presenting at the same time strong morphological variations.A. flavus CBS 120.62 also lost its toxicogenicity after six subcultures.

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References

  1. Armbrecht, B.H., F.A. Hogges, Smith & A.A. Nelson. 1963. Micotoxins I. Studies on aflatoxin derived from contaminated peanut meal and certain strains of Aspergillus flavus. Ass. Off. Chem. J. 46: 805–817.

    Google Scholar 

  2. Davis, N.D., U.L. Diener & V.P. Agnihotri. 1967. Production of aflatoxin B1 and G1 in chemically defined medium by Aspergillus flavus. Mycopathol. Mycol. Appl. 31: 251–256.

    Google Scholar 

  3. De Vogel, P., R. Van Rhee & W.A.A. Koelensmith. 1965. A rapid screening test for aflatoxin-synthetizing Aspergilli of the flavus-oryzae group. J. Appl. Bact. 28: 213–220.

    Google Scholar 

  4. Diener, V.L. & N.D. Davis. 1968. Production of aflatoxins on peanuts under controlled environments. Ist. Int. Congr. Pl. Pathol. London.

  5. Goldbatt, L.A. 1968. Aflatoxin and its control. Economic Botany 22: 51–62.

    Google Scholar 

  6. Goldbatt, L.A. 1975. Quantitative aflatoxin standard SA 14-10. Southern Regional Research Center. Agricultural Research Service. New Orleans, Lousiana 70179, U.S.A.

    Google Scholar 

  7. Hesseltine, C.W., O.L. Shotwell, J.J. Ellis & R.D. Stubblefield. 1966. Aflatoxin formation by Aspergillus flavus Bact. Rev. 30: 795–805.

    Google Scholar 

  8. Jacquet, J. & P. Boutibonnes. 1969. Actions de l'aflatoxines sur les cellules microbiennes. C.R. Soc. Biol. 158: 2.574–2.577.

    Google Scholar 

  9. Jacquet, J. & P. Boutibonnes. 1969. Fluorescence des aflatoxines et des Aspergillus. C.R. Soc. Biol. 169: 2289–2293.

    Google Scholar 

  10. Jacquet, J. & P. Boutibonnes. 1970. Recherche sur les flavotoxines oux mieux flavocumarines. Rev. Inmunol. 39: 245–274.

    Google Scholar 

  11. Kulik, M.M. & C.F. Holaday. 1966. Aflatoxins: a metabolic product of several fungi. Mycopathol. Mycol. Appl. 30: 137–140.

    Google Scholar 

  12. Murakami, H.S. Takase & K. Kuwabara. 1968. Nonproductivity of aflatoxin by Japanese industrial strains of Aspergillus. II.—Production of fluorescent substances in rice Koji, and their identification by absorption spectrum. J. Gen. Appl. Microbiol. 14: 97–110.

    Google Scholar 

  13. Murakami, H., H. Sagawa & S. Takase. 1968. Non-productivity of aflatoxin by Japanese industrial strains of the Aspergillus. III. — Common characteristics of the aflatoxinproducing strains. J. Gen. Appl. Microbiol. 14: 251–262.

    Google Scholar 

  14. Murakami, H. & M. Suzuki. 1970. Mycological differences between the producers and non-producer of aflatoxin of Aspergillus in Herzberg, M. Microorganisms pp. 198–201.

  15. Pons, W.A., A.F. Cucullu, L.S. Lee, J.A. Robertson, A.O. Franz & L.A. Goldbatt. 1966. Determination of aflatoxins in agricultural products: use of aqueous acetone for extraction. J. Ass. Off. Ana. Chem. 49:554–562.

    Google Scholar 

  16. Robertson, J.A., W.A. Pons & L.A. Goldbatt. 1967. Preparation of aflatoxins and determination of their ultraviolet and fluorescent characteristics. J. Agr. Food. Chem. 15: 798–801.

    Google Scholar 

  17. Tuttlebee, J.W. 1975. The Stomache: its use for homogeneization in food microbiology. J. Food Technol. 10: 113–122.

    Google Scholar 

  18. Wildman, J.D., L. Stoloff & R. Jacobs. 1967. Aflatoxin production by a potent Aspergillus flavus Link isolate. Biotechnol. Bioeng. 9: 429–437.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain

    M. A. Calvo

  2. Laboratory of General and Applied Mycology, Instituto ‘Jaime Ferrán’ de Microbiología del Consejo Superior de Investigaciones Científicas, Madrid, Spain

    C. Ramírez

Authors
  1. J. Torres
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  2. J. Guarro
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  3. G. Suarez
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  4. N. Suñe
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  5. M. A. Calvo
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  6. C. Ramírez
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Torres, J., Guarro, J., Suarez, G. et al. Morphological changes in strains of Aspergillus flavus link ex fries and Aspergillus parasiticus speare related with aflatoxin production. Mycopathologia 72, 171–174 (1980). https://doi.org/10.1007/BF00572660

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