Summary
Anti-neuroparsin serum was immunohistochemically tested on brain and/or neurohemal organ from 40 insect species belonging to 13 orders, and from 8 non-insect invertebrate and 5 vertebrate representatives using the peroxidase-antiperoxidase procedure. In insects, immunostaining of only the A1 type of the protocerebral median neurosecretory cells was revealed in all species tested of Odonata. Dictyoptera, Isoptera and Orthoptera and in 2 species from the 9 other orders out of 13 orders tested. No immunostaining was detected in vertebrate and non-insect invertebrate species except in 2 annelid species out of 4 tested. The distribution of neuroparsin-like products in Coelomata appears to be restricted mainly to 4 phylogenetically close insect orders.
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References
Bernstein HG, Eckert M, Penzlin H, Dorn A (1984) Proctolinrelated material in the mouse brain as revealed by immunohistochemistry. Neurosci Lett 45:229–232
Bishop CA, Wine JJ, O'Shea M (1984) Neuropeptide proctolin in postural motoneurons of the crayfish. J Neurosci 4:2001–2009
Bourême D, Girardie J (1986) Présence de protéines à ponts disulfures dans les corpora cardiaca d'insectes. CR Acad Sci Paris 302:655–660
Bourême D, Tamarelle M, Girardic J (1987) Production and characterization of antibodies to neuroparsins A and B isolated from the corpora cardiaca of the locust. Gen Comp Endocrinol 67:169–177
Fournier B, Girardie J (1988) A new function for the locust neuroparsins: stimulation of water reabsorption. J Insect Physiol 4:309–313
Ganagarajah M, Saleuddin ASM (1970) A simple manoeuvre to prevent loss of the sections for performic acid-Victoria blue technique and a comparison of various neurosecretory stains. Can J Zool 48:1457–1458
Girardie J, Bourême D, Girardie A (1986) Production sites of the three neurosecretory proteins characterized in the corpora cardiaca of the migratory locust. Insect Biochem 17:29–36
Holets VR, Hokfelt T, Ude J, Eckert M, Hansen S (1984) Coexistence of proctolin with TRH and 5-HT in the rat CNS. Soc Neurosci Abstr 10:692
Moreau R, Gourdoux L, Girardie J (1988) Neuroparsin: a new energetic neurohormone in the African Locust. Arch Insect Biochem Physiol 8:135–145
Myers CM, Evans PD (1987) An FMR Famide antiserum differentiates between populations of antigens in the brain and retrocerebral complex of the locust Schistocerca gregaria. Cell Tissue Res 250:93–99
Rémy C (1984) Immunochemical relationships between verterbrate and invertebrate neuropeptides. Nova Acta Leopoldina 56:99–116
Sasek CA, Schueler PA, herman WS, Elde RP (1985) An antiserum to locust adipokinetic hormone reveals a novel peptidergic system in the rat central nervous system. Brain Res 343:172–175
Schooneveld H, Romberg-Privee HN, Veenstra JA (1986) Immunocytochemical differentiation between adipokinetic hormone (AKH)-like peptides in neurons and glandular cells in the corpus cardiacum of Locusta migratoria and Periplaneta americana with C-terminal and N-terminal specific antisera to AKH. Cell Tissue Res 243:9–14
Schwarz TL, Lee GMH, Siwicki KK, Standaert DG, Kravitz EA (1984) Proctolin in the lobster: the distribution release and characterization of a likely neurohormone. J Neurosci 11:1300–1311
Starratt AN, Brown BE (1975) Structure of the pentapeptide proctolin, a proposed neurotransmitter in insects. Life Sci 17:1253–1256
Sternberger LA (1986) Immunocytochemistry, 3rd edn. Wiley, New York
Sternberger LA, Hardy JPH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Stone JV, Mordue W, Batley KE, Morris HR (1976) Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flight. Nature 263:207–211
Veenstra JA, Romberg-Privee HM, Schooneveld H (1985) A proctolin-like peptide and its immunocytochemical localization in the colorado potato beetle, Leptinotarsa decemlineata. Cell Tissue Res 240:535–540
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Tamarelle, M., Girardie, J. Immunohistochemical investigation of locust neuroparsin-like substances in several insects, in some other invertebrates, and vertebrates. Histochemistry 91, 431–435 (1989). https://doi.org/10.1007/BF00493831
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DOI: https://doi.org/10.1007/BF00493831