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Sudan Black B: Chemical structure and histochemistry of the blue main components

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Summary

Sudan Black B contains two blue main components, SSB-I and SSB-II. Their chemical structures were determinated by the aid of twodimensional thin-layer chromatography, column chromatography, absorption, IR, mass, H1-NMR, and C13-NMR spectroscopy and were proved by alternate synthesis. SSB-I has been found to be 2,3-dihydro-2,2-dimethyl-4-[(4-phenylazo-l-naphthalenyl)-azo]-lH-perimidine. For SSB-II was confirmed the known structure 2,3-dihydro-2,2-dimethyl-6-[(4-phenylazo-l-naphthalenyl)-azo]-lH-perimidine. Relations of chemical structure of SSB-I and SSB-II to their staining properties are discussed.

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Authors and Affiliations

  1. State Institute for Immune Preparates and Nutrition Media, Klement-Gottwald-Allee 317-321, DDR-112, Berlin, German Democratic Republic

    U. Pfüller, H. Franz & A. Preiß

  2. VEB Chemiekombinat Bitterfeld, Bitterfeld, German Democratic Republic

    U. Pfüller, H. Franz & A. Preiß

Authors
  1. U. Pfüller
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  2. H. Franz
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  3. A. Preiß
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Professor Dr. G. Holle on the occasion on his 65th birthday

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Pfüller, U., Franz, H. & Preiß, A. Sudan Black B: Chemical structure and histochemistry of the blue main components. Histochemistry 54, 237–250 (1977). https://doi.org/10.1007/BF00492246

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  • DOI: https://doi.org/10.1007/BF00492246

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