Article

Archives of oto-rhino-laryngology

, Volume 224, Issue 1, pp 129-134

Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney

  • Jochen SchachtAffiliated withKresge Hearing Research Institute, The University of Michigan

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Summary

Affinity chromatography was used to isolate aminoglycoside receptors from inner ear tissues and kidney. Neomycin was immobilized on glass beads and served as the stationary phase in column chromatography. Fractionation of an organic tissue extract on this matrix demonstrated two components with high affinity for neomycin: phosphatidyl inositol phosphate and phosphatidyl inositol diphosphate. The toxicity of aminoglycosides is explained on the basis of a drug-interaction with these lipids.

Key words

Aminoglycoside ototoxicity Neomycin Drug receptors Affinity chromatography Polyphosphoinositides