, Volume 224, Issue 1-2, pp 129-134

Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney

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Summary

Affinity chromatography was used to isolate aminoglycoside receptors from inner ear tissues and kidney. Neomycin was immobilized on glass beads and served as the stationary phase in column chromatography. Fractionation of an organic tissue extract on this matrix demonstrated two components with high affinity for neomycin: phosphatidyl inositol phosphate and phosphatidyl inositol diphosphate. The toxicity of aminoglycosides is explained on the basis of a drug-interaction with these lipids.

This research was supported by Research Grant NS-13792 and Program Project Grant NS-05785 from the National Institutes of Health