Archives of oto-rhino-laryngology

, Volume 224, Issue 1, pp 129–134

Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney

Authors

  • Jochen Schacht
    • Kresge Hearing Research InstituteThe University of Michigan
Article

DOI: 10.1007/BF00455236

Cite this article as:
Schacht, J. Arch Otorhinolaryngol (1979) 224: 129. doi:10.1007/BF00455236

Summary

Affinity chromatography was used to isolate aminoglycoside receptors from inner ear tissues and kidney. Neomycin was immobilized on glass beads and served as the stationary phase in column chromatography. Fractionation of an organic tissue extract on this matrix demonstrated two components with high affinity for neomycin: phosphatidyl inositol phosphate and phosphatidyl inositol diphosphate. The toxicity of aminoglycosides is explained on the basis of a drug-interaction with these lipids.

Key words

Aminoglycoside ototoxicityNeomycinDrug receptorsAffinity chromatographyPolyphosphoinositides

Copyright information

© Springer-Verlag 1979