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Analysis of the fermentation pathways of clostridia using double labelled glutamade

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Abstract

l-(4-14C, 3-3H)Glutamate was used as a tool to elucidate the pathway of its fermentation. The methylaspartate pathway was found in strains of Clostridium tetanomorphum, C. malenominatum, C. limosum, C. lentoputrescens and C. tetani, whereas the hydroxyglutarate pathway was detected in C. sporosphaeroides. These data were supported by measurement of enzyme activities present in cell-free extracts.

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References

  • Blair, A. H., Barker, H. A.: Assay and purification of (+)-citramalate hydro-lyase components from Clostridium tetanomorphum. J. Biol. Chem. 241, 400–408 (1966)

    Google Scholar 

  • Buckel, W.: The reversible dehydration of (R)-2-hydroxyglutarate to (E)-glutaconate. Eur. J. Biochem. 106, 439–447 (1980)

    Google Scholar 

  • Buckel, W., Barker, H. A.: Two pathways of glutamate fermentation by anaerobic bacteria. J. Bacteriol. 117, 1248–1260 (1974)

    Google Scholar 

  • Buckel, W., Bobi, A.: The enzyme complex citramalate lyase from Clostridium tetanomorphum. Eur. J. Biochem. 64, 255–262 (1976)

    Google Scholar 

  • Elsden, S. R., Hilton, M. G., Waller, J. M.: The end products of the metabolism of aromatic amino acids by Clostridia. Arch. Microbiol. 107, 283–288 (1976)

    Google Scholar 

  • Elsden, S. R., Hilton, M. G.: Volatile acid production from threonine, valine, leucine and isoleucine by Clostridia. Arch. Microbiol. 117, 165–172 (1978)

    Google Scholar 

  • Lienhard, G. E., Rose, I. A.: The stereochemistry of decarboxylation of isocitrate by isocitric acid dehydrogenase. Biochemistry 3, 185–190 (1964)

    Google Scholar 

  • Mosbach, E. H., Phares, E. F., Carson, S. F.: Degradation of isotopically labelled citric, α-ketoglutaric and glutamic acids. J. Biol. Chem. 211, 907–913 (1951)

    Google Scholar 

  • Nakamura, S., Okado, I., Abe, T., Nishida, S.: Taxonomy of Clostridium tetani and related species. J. Gen. Microbiol. 113, 29–35 (1979)

    Google Scholar 

  • Seki, T.: Chromatographic separation of lower fatty acids. J. Biochem. (Tokyo) 45, 855–860 (1958)

    Google Scholar 

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  1. Biochemie I, Fakultät für Biologie und Vorklinische Medizin der Universität Regensburg, Universitätsstr. 31, D-8400, Regensburg, Federal Republic of Germny

    Wolfgang Buckel

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  1. Wolfgang Buckel
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Buckel, W. Analysis of the fermentation pathways of clostridia using double labelled glutamade. Arch. Microbiol. 127, 167–169 (1980). https://doi.org/10.1007/BF00428021

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  • DOI: https://doi.org/10.1007/BF00428021

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