Summary
The cytoplasmic ribosomal proteins from a fission yeast Schizosaccharomyces pombe were analysed by two-dimensional polyacrylamide gel electrophoresis. Seventy-three protein species were identified in the 80S ribosome, and named SP-S1 to SP-S33 and SP-L1 to SP-L40 in the small and large subunits, respectively. Many of these proteins could be correlated to those of Saccharomyces cerevisiae on the basis of their electrophoretic mobilities. Eleven proteins were isolated from the 80S ribosome, and their amino acid compositions were determined. Of these, SP-S6, SP-L1, SP-L12, SP-L15, SP-L17, SP-L27, SP-L36 and SP-L40c and d were sequenced from their amino-termini. SP-S28 and SP-L2 appear to have their amino-termini blocked. These results were compared with the data available for the S. cerevisiae and rat liver ribosomal proteins. The S. cerevisiae counterparts of the eight proteins mentioned above were found to be YS4, YL1, YL10, YL14, YL35, YL40 and YL44c and d, respectively. The rat liver counter-parts of SP-S6, SP-L1, SP-L27 and SP-L40c and d were the rat S6, L4, L37 and P2, respectively. Comparison of the partial sequences of these ribosomal proteins suggests that these two yeasts are relatively far apart, phylogenetically.
Similar content being viewed by others
References
Barnitz JT, Cramer JH, Rownd RH, Cooley L, Söll D (1982) Arrangement of the ribosomal RNA genes in Schizosaccharomyces pombe. FEBS Lett 143:129–132
Coddington A, Fluri R (1977) Characterization of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis. Mol Gen Genet 158:93–100
Erdmann VA, Huysmans E, Vandenberghe A, De Wachter R (1983) Collection of published 5S and 5.8S ribosomal RNA sequences. Nucl Acids Res 11:r105-r133
Gressner AM, Wool IG (1974) The stimulation of the phosphorylation of ribosomal protein S6 by cycloheximide and puromycin. Biochem Biophys Res Commun 60:1482–1490
Higo K, Otaka E (1979) Isolation and characterization of fourteen ribosomal proteins from small subunits of yeast. Biochemistry 18:4191–4196
Higo K, Itoh T, Osawa S (1978) Evolution of ribosomal proteins. In: Matsubara H, Yamanaka T (eds) Evolution of protein molecules. Japan Scientific Societies Press, Tokyo, pp 197–207
Higo K, Itoh T, Kumazaki T, Osawa S (1980) Ribosomal proteins from mesophilic and thermophilic bacteria. In: Osawa S, Ozeki H, Uchida H, Yura T (eds) Genetics and evolution of RNA polymerase, tRNA and ribosomes. University of Tokyo Press, Tokyo, pp 655–666
Higo K, Otaka E, Osawa S (1982) Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: Correlation to Eschericha coli 30S proteins. Mol Gen Genet 185:239–244
Hori H, Itoh T, Osawa S (1982) The phylogenic structure of the metabacteria. Zentralbl Bakteriol Parasitenk Infektionskr Hyg Abt I Orig Reihe C3:18–30
Itoh T (1980) Primary structure of yeast acidic ribosomal protein YP A1. FEBS Lett 114:119–123
Itoh T, Higo K, Otaka E (1979) Isolation and characterization of twenty-three ribosomal proteins from large subunits of yeast. Biochemistry 18:5787–5793
Itoh T, Higo K, Otaka E, Osawa S (1980) Studies on the primary structures of yeast ribosomal proteins. In: Osawa S, Ozeki H, Uchida H, Yura T (eds) Genetics and evolution of RNA polymerase, tRNA and ribosomes. University of Tokyo Press, Tokyo, pp 609–624
Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for finger-printing of ribosomal proteins. Anal Biochem 36:401–412
Komiya H, Miyazaki M, Takemura S (1981) The nucleotide sequence of 5S ribosomal RNA from Schizosaccharomyces pombe. J Biochem (Tokyo) 89:1663–1666
Kruiswijk T, Planta RJ (1975) Further analysis of the protein composition of yeast ribosomes. FEBS Lett 58:102–105
Lin A, Wittmann-Liebold B, McNally J, Wool IG (1982) The primary structure of the acidic phosphoprotein P2 from rat liver 60S ribosomal subunits. J Biol Chem 257:9189–9197
Mao J, Appel B, Schaack J, Sharp S, Yamada H, Söll D (1982) The 5S RNA genes of Schizosaccharomyces pombe. Nucl Acids Res 10:487–500
McConkey EH, Bielka H, Gordon J, Lastick SM, Lin A, Ogata K, Reboud J-P, Traugh JA, Traut RR, Warner JR, Welfle H, Wool IG (1979) Proposed uniform nomenclature for mammalian ribosomal proteins. Mol Gen Genet 169:1–6
Mets LJ, Bogorad L (1974) Two-dimensional polyacrylamide gel electrophoresis: An improved method for ribosomal proteins. Anal Biochem 57:200–210
Ogata K, Terao K (1981) Analytical methods for ribosomal proteins of rat liver 40S and 60S subunits by “three-dimensional” acrylamide gel electrophoresis. In: Moldave K (ed) RNA and protein synthesis. Academic Press, New York, pp 598–610
Otaka E, Kobata K (1978) Yeast ribosomal proteins: I. Characterization of cytoplasmic ribosomal proteins by two-dimensional gel electrophoresis. Mol Gen Genet 162:259–268
Otaka E, Osawa S (1981) Yeast ribosomal proteins: V. Correlation of several nomenclatures and proposal of a standard nomenclature. Mol Gen Genet 181:176–182
Otaka E, Higo K, Osawa S (1982) Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast. Biochemistry 21:4545–4550
Planta RJ, Meyerink JH (1980) Organization of the ribosomal RNA genes in eukaryotes. In: Chambliss G, Craven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes: Structure, function, and Genetics. University Park Press, Baltimore Maryland, pp 871–887
Schaak J, Mao J, Söll D (1982) The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe. Nucl Acids Res 10:2851–2864
Tabata S (1981) Nucleotide sequences of the 5S ribosomal RNA genes and their adjacent regions in Schizosaccharomyces pombe. Nucl Acids Res 9:6429–6437
Warner JR, Gorenstein C (1978) The ribosomal proteins of Saccharomyces cerevisiae. In: Prescott DM (ed) Methods in cell biology, vol 20. Academic Press, New York, pp 45–60
Wittmann-Liebold B, Geissler AW, Lin A, Wool IG (1979) Sequence of the amino-terminal region of rat liver ribosomal proteins S4, S6, S8, L6, L7a, L18, L27, L30, L37, L37a, and L39. J Supramol Struct 12:425–433
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Otaka, E., Higo, Ki. & Itoh, T. Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe . Molec. Gen. Genet. 191, 519–524 (1983). https://doi.org/10.1007/BF00425772
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425772