Archives of Microbiology

, Volume 129, Issue 1, pp 19-22

First online:

Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe

  • Hirokazu InoueAffiliated withDepartment of biology, Faculty of Science, Osaka City University
  • , Chikashi ShimodaAffiliated withDepartment of biology, Faculty of Science, Osaka City University

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Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.

Key words

Germination Glycogen Outgrowth Schizosaccharomyces pombe Spore Trehatase Trehalose Yeast