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Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C

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Abstract

A novel type of bacterium has been isolated from various geothermally heated locales on the sea floor. The organisms are strictly anaerobic, rod-shaped, fermentative, extremely thermophilic and grow between 55 and 90°C with an optimum of around 80°C. Cells show a unique sheath-like structure and monotrichous flagellation. By 16S rRNA sequencing they clearly belong to the eubacteria, although no close relationship to any known group could be detected. The majority of their lipids appear to be unique in structure among the eubacteria. Isolate MSB8 is described as Thermotoga maritima, representing the new genus Thermotoga.

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References

  • Albersheim P, Nevins DJ, English PD, Karr A (1967) A method for the analysis of sugars in plant cell-wall polysaccharides by gas liquid chromatography. Carbohyd Res 5:340–345

    Google Scholar 

  • Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32:781–791

    Google Scholar 

  • Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: Reevaluation of a unique biological group. Microbiol Rev 43:260–296

    Google Scholar 

  • Bergmeyer HU (1974) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim

    Google Scholar 

  • Brock TD (1978) Thermophilic microorganisms and life at high temperatures. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Burgess RR (1976) Purification and properties of E. coli RNA polymerase. In: Losick R, Chamberlin M (eds) RNA Polymerase. Cold Spring Harbor Lab, Cold Spring Harbor, New York

    Google Scholar 

  • Eisenberg F (1974) Gaschromatographic assay of iduronic and glucuronic acids as aldonic acid butaneboronates. Anal Biochem 60:181–187

    Google Scholar 

  • Gottschalk G (1979) Bacterial metabolism. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Heinen W, Lauwers AM (1981) Growth of bacteria at 100°C and beyond. Arch Microbiol 129:127–128

    Google Scholar 

  • Huber H, Thomm M, König H, Thies G, Stetter KO (1982) Methanococcus thermolithotrophicus, a novel thermophilic lithotrophic methanogen. Arch Microbiol 132:47–50

    Google Scholar 

  • Humphries P, McConell DJ, Gordon RJ (1973) A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid dependent ribonucleic acid polymerase. Biochem J 133:201–203

    Google Scholar 

  • Kessel M, Klink F (1982) Identification and comparison of eighteen archaebacteria by means of the diphtheria toxin reaction. Zbl Bakt Hyg Abt I Orig C 3:140–148

    Google Scholar 

  • Klein RA, Hazlewood GP, Kemp P, Dawson RMC (1979) A new series of long-chain dicarboxylic acids with dimethyl branching found as major components of the lipids of Butyrivibrio spp. Biochem J 183:691–700

    Google Scholar 

  • König H, Skorko R, Zillig W, Reiter WD (1982) Glycogen in thermoacidophilic archaebacteria of the genera Sulfolobus, Thermoproteus, Desulfurococcus and Thermococcus. Arch Microbiol 132:297–303

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophate T 4. Nature 227:680–685

    Google Scholar 

  • Langworthy TA (1982) Lipids of Thermoplasma. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 88. Academic Press, New York, pp 396–406

    Google Scholar 

  • Langworthy TA (1985) Lipids of archaebacteria. In: Woese CR, Wolfe RW (eds) The bacteria, vol VIII. Academic Press Inc, Orlando, pp 459–497

    Google Scholar 

  • Langworthy TA, Holzner G, Zeikus JG, Tornabene TG (1983) Iso- and anteiso-branched glycerol diethers of the thermophilic anaerobe Thermodesulfotobacterium commune. System Appl Microbiol 4:1–17

    Google Scholar 

  • Larson DM, Setsinger DC, Waibel PE (1971) Procedure for the determination of d-amino acids. Anal Biochem 39:395–401

    Google Scholar 

  • Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    Google Scholar 

  • Mirault ME, Scherrer K (1971) Isolation of preribosomes from Hela cells and their characterization by electrophoresis on uniform and exponential-gradient-polyacrylamide gels. Eur J Biochem 23:372–386

    Google Scholar 

  • Rauen HM (1964) Biochemisches Taschenbuch, 1. Teil. Springer, Berlin Heidelberg Göttingen

    Google Scholar 

  • Rhuland LE, Work E, Denman RF, Hoare DS (1955) The behaviour of the isomers of α, ε-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77:4844–4846

    Google Scholar 

  • Riva S, Fietta A, Silvestri LG (1972) Mechanism of action of a rifamycin derivative (AF/013) which is active on the nucleic acid polymerases insensitive to rifampicin. Biochem Biophys Res Commun 49:1263–1271

    Google Scholar 

  • Schaller H, Nüsslein C, Bonhoeffer J, Kurz C, Nietschmann J (1972) Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns. Eur J Biochem 26:474–481

    Google Scholar 

  • Schleifer KH, Kandler O (1967) Zur chemischen Zusammensetzung der Zellwand der Streptokokken. I. Die Aminosäuresequenz des Mureins von Streptococcus thermophilus und Streptococcus faecalis. Arch Mikrobiol 57:335–364

    Google Scholar 

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407–477

    Google Scholar 

  • Sleytr UB, Messner P (1983) Crystalline surface layers on bacteria. Ann Rev Microbiol 37:311–339

    Google Scholar 

  • Stetter KO (1982) Ultrathin mycelia-forming organisms from submarine volcanic areas having an optimum growth temperature of 105°C. Nature 300:258–260

    Google Scholar 

  • Stetter KO (1985) Extrem thermophile Bakterien. Naturwissenschaften 72:291–301

    Google Scholar 

  • Stetter KO, Zillig W (1985) Thermoplasma and the thermophilic sulfur-dependent archaebacteria. In: Wolfe RS, Woese CR (eds) The bacteria, vol VIII. Academic Press, New York, pp 85–170

    Google Scholar 

  • Stetter KO, Thomm M, Winter J, Wildgruber G, Huber H, Zillig W, Janecovic D, König H, Palm P, Wunderl S (1981) Methanothermus fervidus, sp. nov., a novel extremely thermophilic methanogen isolated from an Icelandic hot spring. Zbl Bakt Hyg Abt I Orig C 2:166–178

    Google Scholar 

  • Stetter KO, König H, Stackebrandt E (1983) Pyrodictium gen. nov., a new genus of submarine disc-shaped sulphur-reducing archaebacteria growing optimally at 105°C. Syst Appl Microbiol 4:535–551

    Google Scholar 

  • Thomm M, Stetter KO (1985) Transcription in methanogens. Evidence for specific in vitro transcription of the purified DNA-dependent RNA polymerase of Methanococcus thermolithotrophicus. Eur J Biochem 149:345–351

    Google Scholar 

  • Williams WJ (1979) Handbook of anion determination. Butterworths, London, pp 570–572

    Google Scholar 

  • Zabel HP, König H, Winter J (1985) Emended description of Methanobacterium thermophilicum, Rivard and Smith, and assignment of new isolates to this species. Syst Appl Microbiol 6:72–78

    Google Scholar 

  • Zillig W, Stetter KO, Wunderl S, Schulz W, Priess H, Scholz I (1980) The Sulfolobus-“Caldariella”-group: Taxonomy on the basis of the structure of DNA-dependent RNA polymerases. Arch Microbiol 125:259–269

    Google Scholar 

  • Zillig W, Stetter KO, Schäfer W, Janekovic D, Wunderl S, Holz I, Palm P (1981) Thermoproteales: A novel type of extremely thermoacidophilic anaerobic archaebacteria isolated from Icelandic solfataras. Zbl Bakt Hyg Abt I Orig C 2:205–227

    Google Scholar 

  • Zillig W, Holz I, Janekovic D, Schäfer W, Reiter WD (1983) The archaebacterium Thermococcus celer represents a novel genus within the thermophilic branch of the archaebacteria. Syst Appl Microbiol 4:88–94

    Google Scholar 

  • Zillig W, Stetter KO, Schnabel R, Thomm M (1985) DNA-dependent RNA polymerases of the archaebacteria. In: Woese CR, Wolfe RS (eds) The Bacteria, vol VIII. Academic Press Inc, Orlando, pp 499–524

    Google Scholar 

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Dedicated to Otto Kandler on the occasion of his 65th birthday

Present addresses: University of South Dakota, Vermillion, USA; University of Illinois, Urbana, USA; Universität für Bodenkultur, Wien, Austria

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Huber, R., Langworthy, T.A., König, H. et al. Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch. Microbiol. 144, 324–333 (1986). https://doi.org/10.1007/BF00409880

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  • DOI: https://doi.org/10.1007/BF00409880

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