Summary
A high performance liquid chromatographic method for the determination of hippuric and m-(p-)methylhippuric acids in urine, metabolites of toluene and m-(p-)xylene, is described. A stainless-steel column packed with octadecyl silanized silica gel was used and the mobile phase was a mixed solution of methanol/water/acetic acid (20/80/0.2). The method is simple and specific. Urine can be analyzed directly, without solvent extraction or pretreatment. The method has a lower detection limit of 0.2 μg on column. All the analysis and quantitative determination can be performed within about 30 min. for samples containing m-(p-)methylhippuric acid.
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References
Ogata, M., Tomokuni, K., Takatsuka, T.: Quantitative determination in urine of hippuric acid and m- or p-methylhippuric acid, metabolites of toluene and m- or p-xylene, Brit. J. Industr. Med. 26, 330–334 (1969)
Ogata, M., Sugihara, R.: An improved method of direct colorimetric determination of hippuric acid in urine, Jap. J. Ind. Health (Jap.) 18, 40–41 (1976)
Tomokuni, K., Ogata, M.: Direct colorimetric determination of hippuric acid in urine, Clin. Chem. 18, 349–351 (1972)
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Ogata, M., Sugihara, R. & Kira, S. Quantitative determination of urinary hippuric acid and m- or p-Methylhippuric Acid as Indices of toluene and m- or p-Xylene exposure by high performance liquid chromatography. Int. Arch Occup Environ Heath 39, 199–206 (1977). https://doi.org/10.1007/BF00409366
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DOI: https://doi.org/10.1007/BF00409366