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Bacterial assimilation of d- and l-2-chloropropionates and occurrence of a new dehalogenase

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Abstract

The occurrence of a new bacterial dehalogenase acting on both the optical isomers of 2-halogenated alkanoic acids was demonstrated. When the haloalkanoic acid-utilizing bacteria were screened in a medium containing dl-2-chloropropionate as a sole carbon source, two types of bacteria were isolated: (1) a few strains utilizing both d- and l-isomers of 2-chloropropionate and (2) strains utilizing only the l-isomer. A dehalogenating enzyme was obtained from the cells of Pseudomonas sp. which is able to utilize both isomers. The crude enzyme catalyzed the dehalogenation of d- and l-2-chloropropionates to yield l- and d-isomers of lactate, respectively. The enzyme showed the same pH optimum and heat inactivation rate for the d- and l-isomers. Apparent K m values for d- and l-2-chloropropionates were 4.5 and 1.0 mM, respectively. The enzyme acted specifically on 2-haloalkanoic acids. Activity staining of disc-gels electrophoresed witg the crude enzyme preparation showed that the dehalogenation of d- and l-2-chloropropionates, monochloroacetate, dichloroacetate, 2,2-dichloropropionate, and dl-2-chlorobutyrate is due to a single protein.

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Abbreviations

MCA:

monochloroacetic acid

DCA:

dichloroacetic acid

TCA:

trichloroacetic acid

2 MCPA:

2-monochloropropionic acid

22 DCPA:

2,2-dichloropropionic acid

3 MCPA:

3-monochloropropionic acid

2 MCBA:

2-monochlorobutyric acid

3 MCBA:

3-monochlorobutyric acid

4 MCBA:

4-monochlorobutyric acid

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Motosugi, K., Esaki, N. & Soda, K. Bacterial assimilation of d- and l-2-chloropropionates and occurrence of a new dehalogenase. Arch. Microbiol. 131, 179–183 (1982). https://doi.org/10.1007/BF00405875

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  • DOI: https://doi.org/10.1007/BF00405875

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