Summary
The ultrastructure of organelles appearing in the early typical and atypical spermatids, and the nutritive cells of Cipangopaludina malleata has been examined by a Siemens' electron microscope Elmiskop I.
Mitochondria appearing in the early typical spermatid have doughnut-like profiles in which the internal ridges appear as triple-layered membranes arranged radially and extending into the interior of the organelle without reaching the other side. Each membrane 40–60 Å in width, separated by a clear interspace 60–90 Å wide, is characterized by a porous structure 20–30 Å in diameter which suggests a filtration apparatus for enzymes.
Walls of the flattened saccules consisting the Golgi apparatus are calculated 35–60 Å thick, in which an electron-lucent, porous structure about 30 Å wide has been revealed.
The smooth-surfaced endoplasmic reticulum is bordered by a triple-layered membrane consisting of two opaque layers with a less opaque interspace 20–30 Å wide. The outer membrane ca. 15 Å wide presents a more linear appearance than the dotted arrangement of the inner membrane 20–25 Å thick.
The plasma membrane is composed of a triple-layered structure where two dense lines 15 Å wide are separated by a layer 20–30 Å thick of less density.
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References
Beams, H.W., and T.N. Tahmistan: Structure of the mitochondria in the male germ cells of Helix as revealed by the electron microscope. Exp. Cell Res. 6, 87–93 (1954).
Beams, H.W., T.N. Tahmisian, R.L. Devine and E. Anderson: Electron microscope studies on the dictyosomes and acroblasts in the germ cells of the cricket. J. biophys. biochem. Cytol. 2, No 4, Suppl. 123–128 (1956).
Dalton, A.J., and M.D. Felix: Studies on the Golgi substance of the epithelial cells of the epididymis and duodenum of the mouse. Amer. J. Anat. 92, 277–305 (1953).
: A comparative study of the Golgi complex. J. biophys. biochem. Cytol. 2, No 4, Suppl. 79–84 (1956).
Danielli, J.F.: The cell surface and cell physiology. In: Cytology and cell physiology (G. Bourne, ed.). London: Oxford University Press 1942.
Ekholm, R., and F.S. Sjöstrand: The ultrastructural organization of the mouse thyroid gland. J. Ultrastructure Res. 1, 178–199 (1957).
Freeman, J.A.: The ultrastructure of the double membrane systems of mitochondria. J. biophys. biochem. Cytol. 2, No 4, Suppl. 353–354 (1956).
Frey-Wyssling, A.: Submicroscopic Morphology of Protoplasm, p. 199. Amsterdam-Houston-London-New York: Elsevier Publishing Comp. 1953.
Gatenby, J.B., A.J. Dalton and M.D. Felix: The contractile vacuole of parazoa and protozoa, and the Golgi apparatus. Nature (Lond.) 176, 301–302 (1955).
Grassé, P.P.: L'appareil parabasal et l'appareil de Golgi sont un même organite. Leur ultrastructure, leurs de sécrétion. C. R. Acad. Sci. (Paris) 242, 858–861 (1956 a).
: L'appareil de Golgi des protozoaires et son ultrastructure comparée à celle des métazoaires. Electron Microscopy. Proc. of the Stockholm Conference, p. 143–145. Stockholm: Almqvist & Wiksell 1956b.
: Ultrastructure, polarité et reproduction de l'apparareil de Golgi. C. R. Acad. Sci. (Paris) 243, 1278–1281 (1957).
Grassé, P.P., and N. Carasso: Ultra-structure of the Golgi apparatus in protozoa and metazoa (somatic and germinal cells). Nature (Lond.) 179, 31–33 (1957).
Grassé, P.P., N. Carasso and P. Favard: Les dictyosomes (appareil de Golgi) et leur ultra-structure. C. R. Acad. Sci. (Paris) 241, 1243–1245 (1955).
Lehninger, A.L.: The organized respiratory activity of isolated rat liver mitochondria, in enzymes and enzyme systems, their state in nature (J.T. Edsall, ed.). Cambridge, Mass.: Havard Univ. Press 1951.
Noirothtimothée, C.: L'ultrastructure de l'appareil de Golgi des Infusoires Ophryoscolecidae. C. R. Acad. Sci. (Paris) 244, 2847–2849 (1957).
Palade, G.E.: The fine structure of mitochondria. Anat. Rec. 114, 427–452 (1952).
: An electron microscope study of the mitochondrial structure. J. Histochem. Cytochem. 1, 188–211 (1953).
: A small particulate component of the cytoplasm. J. biophys. biochem. Cytol. 1, 59–68 (1955).
: The endoplasmic reticulum. J. biophys. biochem. Cytol. 2, No 4, Suppl. 85–98 (1956).
Palay, S.L., and G. E. Palade: The fine structure of neuron. J. biophys. biochem. Cytol. 1, 69–88 (1955).
Powers, E.L., C.F. Ehret and L.E. Roth: Mitochondrial structure in Paramecium as revealed by electron microscopy. Biol. Bull. 108, 182–195 (1955).
Rhodin, J.: Correlation of ultrastructural organization and function in normal and experimentally changed proximal convoluted tubule cells of the mouse kidney. Karolinska Institutet, Stockholm, Aktiebolaget Godvil, 1954.
Robertson, J. D.: New observations on the ultrastructure of the membrane of frog peripheral nerve fibers. J. biophys. biochem. Cytol. 3, 1043–1048 (1957).
: The ultrastructure of Schmidt-Lanterman clefts and related shearing defects of the myelin sheath. J. biophys. biochem. Cytol. 4, 39–16 (1958).
Roth, L.E.: Aspects of ciliary fine structure in Euplotes patella. J. biophys. biochem. Cytol. 2, No 4, Suppl. 235–240 (1956).
Schneider, W.C., and G. H. Hogeboom: Chemical studies of mammalian tissues; the isolation of cell components by differential centrifugation; a review. Cancer. Res. 11, 1–22 (1950).
Sedar, A.W., and K. R. Porter: The fine structure of cortical components of Paramecium multimicronucleatum. J. biophys. biochem. Cytol. 1, 583–604 (1955).
Sedar, A.W., and M.A. Rudzinska: Mitochondria of protozoa. J. biophys. biochem. Cytol. 2, No 4, Suppl. 331–336 (1956).
Sjöstrand, F.S.: Electron microscopy of mitochondria and cytoplasmic double membranes. Nature (Lond.) 171, 30–32 (1953).
Yasuzumi, G., W. Fujimura and H. Ishida: Spermatogenesis in animals as revealed by electron microscopy. V. Spermatid differentiation of Drosophila and grasshopper. Exp. Cell. Res. 14, 268–285 (1958).
Yasuzumi, G., and H. Ishida: Spermatogenesis in animals as revealed by electron microscopy. II. Submicroscopic structure of developing spermatid nuclei of grasshopper. J. biophys. biochem. Cytol. 3, 663–668 (1957).
Yasuzumi, G., and H. Tanaka: Spermatogenesis in animals as revealed by electron microscopy. VI. Researches on the spermatozoon-dimorphism in a pond snail, Cipangopaludina malleata. J. biophys. biochem. Cytol. 4, 621–632 (1958).
Zetterqvist, H.: The ultrastructural organization of the columnar absorbing cells of the mouse jejunum. Thesis. Karolinska Institutet, Stockholm, 1956.
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The electron micrographs for the present studies were taken with the Siemens electron microscope, model Elmiskop I, at the Anatomical Institute of Kiel University, Germany. The one of the authors, G. Yasuzumi is deeply grateful to Prof. Dr. W. Bargmann and Dr. A. Knoop for the privilege of using this instrument and other equipments in the Laboratory.
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Yasuzumi, G., Tanaka, H., Tezuka, O. et al. The ultrastructure of organelles appearing in spermatids and nutritive cells of Cipangopaludina malleata . Z.Zellforsch 50, 632–643 (1959). https://doi.org/10.1007/BF00381675
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DOI: https://doi.org/10.1007/BF00381675