International Archives of Occupational and Environmental Health

, Volume 39, Issue 2, pp 103–111

Incubation of 14C-trichloroethylene vapor with rat liver microsomes: Uptake of radioactivity and covalent protein binding of metabolites


  • H. M. Bolt
    • Institut für Toxikologie der Universität
  • A. Buchter
    • Institut und Poliklinik für Arbeits- und Sozialmedizin der Universität
  • L. Wolowski
    • Institut für Toxikologie der Universität
  • D. L. Gil
    • Departmento de Bioquimica, Facultad de MedicinaUniversidad de Chile
  • W. Bolt
    • Institut und Poliklinik für Arbeits- und Sozialmedizin der Universität

DOI: 10.1007/BF00380890

Cite this article as:
Bolt, H.M., Buchter, A., Wolowski, L. et al. Int Arch Occup Environ Health (1977) 39: 103. doi:10.1007/BF00380890


Microsomal uptake irreversible protein binding of labelled trichloroethylene was measured following incubation with rat liver microsomes in an all-glass vacuum system.

If the cofactor for oxidative metabolism, NADPH, is not added, the gaseous trichloroethylene rapidly equilibrates with the microsomal suspension. Addition of NADPH results in a further uptake of 14C-trichloroethylene from the gas phase, linearly with time, which is due to enzymic metabolism. This part of uptake is inhibited by some arylimidazoles and 1.2.3-benzothiadiazoles. The compounds of greatest inhibitory potency were 6-chloro-1.2.3-benzothiadiazole and 5,6-dimethyl-1.2.3-benzothiadiazole. Part of.the metabolites of 14C-trichloroethylene formed by rat liver microsomes were irreversibly bound to microsomal protein, amounting up to 1 nmol per mg microsomal protein per hour. Model experiments on uptake of 14C-trichloroethylene from the gas phase by albumin solutions and liposomal suspensions (from lecithin) showed a rapid equilibration of trichloroethylene also with these systems. Comparison with previous analogous data on vinyl chloride revealed an about 10 times higher affinity of trichloroethylene to albumin and lipid, consistent with the behaviour of both compounds in the rat liver microsomal system.

Key words

TrichloroethyleneVinyl chlorideRat liver microsomesCovalent protein binding of trichloroethyleneAlbuminLecithin liposomes
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© Springer-Verlag 1977