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In vitro biosynthesis of the C-glycosidic bond in aloin

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Abstract

Biosynthetic studies with cell-free extracts from Aloe arborescens Mill. demonstrate the transfer of the glucose moiety from UDP-glucose to aloe emodin anthrone, forming the C-glycosidic linkage in the anthracene derivative aloin. The pH-dependence and the specificity of UDP-glucose and aloe emodin anthrone for the biosynthesis of the C-glycosidic bond in aloin are shown.

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Abbreviations

ADP-Glc:

adenosine-5′-diphosphate glucose

AEA:

aloe emodin anthrone (1,8-dihydroxy-3-(hydroxymethyl)-9(10 H)-anthracenone)

CoASAc:

acetyl coenzyme A

GDP-Glc:

guanosine-5′-diphosphate glucose

Glc:

glucose

Glc-1-P:

glucose-1-phosphate

HPLC:

high performance liquid chromatography

TLC:

thin layer chromatography

UDP-Gal:

uridine-5′-diphosphate galactose

UDP-Glc:

uridine-5′-diphosphate glucose

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Authors and Affiliations

  1. Fakultät für Chemie und Pharmazie, Universität Regensburg, Universitätsstraße 31, D-8400, Regensburg, Federal Republic of Germany

    M. Grün & G. Franz

Authors
  1. M. Grün
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  2. G. Franz
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Grün, M., Franz, G. In vitro biosynthesis of the C-glycosidic bond in aloin. Planta 152, 562–564 (1981). https://doi.org/10.1007/BF00380828

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  • DOI: https://doi.org/10.1007/BF00380828

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