Bioprocess Engineering

, Volume 7, Issue 5, pp 229-234

First online:

Optimization of fed-batch culture of hybridoma cells using dynamic programming: single and multi feed cases

  • M. de TremblayAffiliated withEcole Polytechnique de Montréal
  • , M. PerrierAffiliated withPulp and Paper Research Institute
  • , C. ChavarieAffiliated withEcole Polytechnique de Montréal
  • , J. ArchambaultAffiliated withBiotechnology Research Institute

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The optimization of fed-batch culture of hybridoma cells is accomplished on a mathematical model using dynamic programming. Optimal feed trajectories are found using a seventh order model for a single feed stream containing both glucose and glutamine and for two separate feed streams of glucose and glutamine. Compared to a constant feed rate, optimal trajectories can improve the final MAb concentration by 11 % for the single feed case and by 20% for the multifeed case. Higher MAb concentrations can be expected for fed-batch optimization with feed enriched in nutrients.