Bioprocess Engineering

, Volume 7, Issue 5, pp 229–234

Optimization of fed-batch culture of hybridoma cells using dynamic programming: single and multi feed cases


  • M. de Tremblay
    • Ecole Polytechnique de Montréal
  • M. Perrier
    • Pulp and Paper Research Institute
  • C. Chavarie
    • Ecole Polytechnique de Montréal
  • J. Archambault
    • Biotechnology Research Institute

DOI: 10.1007/BF00369551

Cite this article as:
de Tremblay, M., Perrier, M., Chavarie, C. et al. Bioprocess Engineering (1992) 7: 229. doi:10.1007/BF00369551


The optimization of fed-batch culture of hybridoma cells is accomplished on a mathematical model using dynamic programming. Optimal feed trajectories are found using a seventh order model for a single feed stream containing both glucose and glutamine and for two separate feed streams of glucose and glutamine. Compared to a constant feed rate, optimal trajectories can improve the final MAb concentration by 11 % for the single feed case and by 20% for the multifeed case. Higher MAb concentrations can be expected for fed-batch optimization with feed enriched in nutrients.

Copyright information

© Springer-Verlag 1992