Abstract
The nuclear gene MRP-L13 of Saccharomyces cerevisiae, which codes for the mitochondrial ribosomal protein YmL13, has been cloned and characterized. It is a single-copy gene residing on chromosome XI. Its nucleotide sequence was found to be identical to that of the previously reported ORF YK105. A comparison of the predicted protein sequence of the MRP-L13 gene product and the actual N-terminal amino-acid sequence of the isolated YmL13 protein indicated that the mature protein is preceded by a mitochondrial signal peptide of 86 amino-acid residues, which is the longest among all known mitochondrial ribosomal proteins of S. cerevisiae. No sequence similarity was found to any other ribosomal protein in the current databases. The transcription of MRP-L13 was found to be repressed in the presence of glucose. Its protein product is not strictly essential for mitochondrial functions, but disruption of the gene by insertion of LEU2 noticeably affected cellular growth on non-fermentable carbon sources.
Similar content being viewed by others
References
Attardi G, Schatz G (1988) Annu Rev Cell Biol 4: 289–333
Biteau N, Fremaux C, Hebrard S, Menara A, Aigle M, Crouzet M (1992) Yeast 8: 61–70
Boguta M, Dmochowska A, Borsuk P, Wrobel K, Gargouri A, Lazowska J, Slonismki PP, Szczesniak B, Kruszewska A (1992) Mol Cell Biol 12: 402–412
Carle GF, Olson MV (1986) Proc Natl Acad Sci USA 82: 3756–3760
Cavener DR, Ray SC (1991) Nucleic Acids Res 19: 3185–3192
Dang H, Ellis SR (1990) Nucleic Acids Res 19: 6895–6901
Davis SC, Tzagoloff A, Ellis SR (1992) J Biol Chem 267: 5508–5514
Düsterhöft A, Philippsen P (1992) Yeast 8: 749–759
Fearon K, Mason TL (1988) Mol Cell Biol 8: 3636–3646
Fearon K, Mason TL (1992) J Biol Chem 267: 5162–5170
Graack HR (1990) PhD thesis, Freie Universität, Berlin
Graack HR, Grohmann L, Kitakawa M (1991) Biochimie 73: 838–844
Graack HR, Grohmann L, Kitakawa M, Schäfer KL, Kruft V (1992) Eur J Biochem 206: 373–380
Gray MW (1989) Trends Genet 5: 294–299
Grohmann L, Graack HR, Kitakawa M (1989) Eur J Biochem 183: 155–160
Grohmann L, Graack HR, Kruft V, Choli T, Goldschmidt-Reisin S, Kitakawa M (1991) FEBS Lett 284: 51–56
Haffter P, Fox TD (1992) Mol Gen Genet 235: 64–73
Haffter P, McMullin TW, Fox TD (1991) Genetics 127: 319–326
Harrer R, Schwank S, Schüller H-J, Schweizer E (1993) Curr Genet 24: 136–140
Hinnebusch AG, Liebman SW (1991) Protein synthesis and translational control in Saccharomyces cerevisiae. In: Broach JR, Pringle JR, Jones EW (eds) The molecular and cellular biology of the yeast Saccharomyces vol 1. Genome dynamics, protein synthesis, and energetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Hultman T, Bergh S, Moks T, Uhlen M (1991) Bio-Techniques 10: 84–93
Isono K (1984) Nucleic Acids Res 12: 101–112
Kang W, Matsushita Y, Grohmann L, Graack HR, Kitakawa M, Isono K (1991a) J Bacteriol 173: 4013–4020
Kang W, Matsushita Y, Isono K (1991b) Mol Gen Genet 225: 474–482
Kitakawa M, Isono K (1991) Biochimie 73: 813–825
Kitakawa M, Grohmann L, Graack HR, Isono K (1990) Nucleic Acids Res 18: 1521–1529
Matshushita Y, Kitakawa M, Isono K (1989) Mol Gen Genet 219: 119–124
McMullin TW, Haffter P, Fox TD (1990) Mol Cell Biol 10: 4590–4595
Myers AM, Crivellone MD, Tzagoloff A (1987) J Biol Chem 262: 3388–3397
Pan C, Sirum-Connolly K, Mason TL (1993) Essential features of the peptidyl transferase center in the yeast mitochondrial ribosome. In: Nierhaus KH, Franceschi F, Subramanian AR, Erdmann VA, Wittmann-Liebold B (eds) The translational apparatus. Plenum Press, New York London
Partaledis JA, Mason TL (1988) Mol Cell Biol 8: 3647–3660
Proudfoot N (1991) Cell 64: 671–674
Sambrook J, Fritsch EF, Maniatis T (1989) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Struhl K (1987) Cell 49: 295–297
Subramanian AR (1993) Trends Biochem Sci 18: 177–181
Thomas PS (1980) Proc Natl Acad Sci USA 77: 5201–5205
Tzagoloff A, Myers AM (1986) Annu Rev Biochem 55: 249–285
Zaret KS, Sherman F (1982) Cell 28: 563–573
Author information
Authors and Affiliations
Additional information
Communicated by P. P. Slonimski
Rights and permissions
About this article
Cite this article
Grohmann, L., Kitakawa, M., Isono, K. et al. The yeast nuclear gene MRP-L13 codes for a protein of the large subunit of the mitochondrial ribosome. Curr Genet 26, 8–14 (1994). https://doi.org/10.1007/BF00326298
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00326298