Summary
A simple, nondestructive kanamycin spraying assay for detecting neomycin phosphotransferase II activity in tomato has been developed. This assay does not require the use of tissue culture or biochemical methods, but allows transgenic and non-transgenic tomato plants to be distinguished directly at the plant level in the green-house. Its potential applications in large-scale genetic analyses are discussed.
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Chyi Y-S, Jorgensen RA, Goldstein D, Tanksley SD, LoaizaFigueroa F (1986) Locations and stability of Agrobacterium mediated T-DNA insertions in the Lycopersicon genome. Mol Gen Genet 204:64–69
Fraley RT, Rogers SG, Horsch RB (1986) Genetic transformation in higher plants. CRC Crit Rev Plant Sci 4:1–46
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of the Vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180
Horsch RB, Fraley RT, Sanders PR, Lloyd A, Hoffmann N (1984) Inheritance of functional foreign genes in plants. Science 223:496–498
Horsch RB, Fry JE, Hoffmann NL, Wallroth M, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1232
Jorgensen RA (1987) Synthetic linkages and genetic stability in transgenic plants: Implications for breeding. In: Nevins DJ, Jones RA (eds) Tomato biotechnology. Alan Liss, New York, pp 179–188
Koornneef M, Hanhart C, Jongsma M, Toma I, Weide R, Zabel P, Hille J (1986) Breeding of a tomato genotype readily accessible to genetic manipulation. Plant Sci 45:201–208
Koornneef M, Jongsma M, Weide R, Zabel P, Hille J (1987) Transformation of tomato. In: Nevins DJ, Jones RA (eds) Tomato biotechnology. Alan Liss, New York, pp 169–178
McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Rep 5:81–84
McDonnell RE, Clark RD, Smith WA, Hinchee MA (1987) A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues. Plant Mol Bio Rep 5:380–386
Reiss B, Sprengel R, Will H, Schaller H (1984) A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts. Gene 30:211–218
Reynaerts A, De Block M, Hernalsteens J-P, Van Montagu M (1988) Selectable and screenable markers. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer, Dordrecht, Sect A9 pp 1–16
Rick CM (1982) Tomato Genet Coop Rep 32:3–7
Schreier PH, Seftor EA, Schell J, Bohnert HJ (1985) The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J 4:25–32
Singleton W, Jones D (1930) Heritable characters of maize. 35. Male sterile. J Hered 21:266–268
Van den Elzen P, Lee KY, Townsend J, Bedbrook J (1985) Simple binary vectors for DNA transfer to plant cells. Plant Mol Biol 4:149–154
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Communicated by G. Wenzel
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Weide, R., Koornneef, M. & Zabel, P. A simple, nondestructive spraying assay for the detection of an active kanamycin resistance gene in transgenic tomato plants. Theoret. Appl. Genetics 78, 169–172 (1989). https://doi.org/10.1007/BF00288794
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DOI: https://doi.org/10.1007/BF00288794