Summary
The remaining unmapped genes encoding known functions of the pyrimidine salvage pathways have been located on the chromosome of Salmonella typhimurium. The gene, cod, encoding cytosine deaminase, udk, encoding uridine kinase and tdk, encoding thymidine kinase were located at 108, 69, and 53 min respectively. The following gene orders were established: serA-metC-argE-cod-strA; cdd-metG-udk-his; and pyrF-cysB-trp-tdk-his. The gene tdk lies in that region of the S. typhimurium chromosome which is inverted with respect to E. coli; its mapping further defines the limits of the inverted segment.
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Beck, C. F., Ingraham, J. L.: Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. Molec. gen. Genet. 111, 303–316 (1971).
Beck, C. F., Ingraham, J. L., Neuhard, J., Thomassen, E.: Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium. J. Bact. (1972) (in press).
Cohen, S. S., Flaks, J. G., Barner, H. D., Loeb, M. R., Lichtenstein, J.: The mode of action of 5-fluoro-uracil and its derivatives. Proc. nat. Acad. Sci. (Wash.) 44, 1004–1012 (1958).
Eisenstark, A., Eisenstark, R., Cunningham, S.: Genetic analysis of thymineless (thy) mutants of Salmonella typhimurium. Genetics 58, 493–506 (1968).
Fink, G. R., Roth, J. R.: Histidine regulatory mutants in Salmonella typhimurium. J. molec. Biol. 33, 547–557 (1968).
Igarashi, K., Higara, S., Yura, T.: A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage 80. Genetics 57, 643–654 (1967).
O'Donovan, G. A., Neuhard, J.: Pyrimidine metabolism in microorganisms. Bact. Rev. 34, 278–343 (1970).
Sanderson, K. E.: Current linkage map of Salmonella typhimurium. Bact. Rev. 34, 176–193 (1970).
Sanderson, K. E., Hall, C. A.: F-prime factors of Salmonella typhimurium and an inversion between S. typhimurium and Escherichia coli. Genetics 64, 215–228 (1970).
Taylor, A. L.: Current linkage map of Escherichia coli. Bact. Rev. 34, 155–175 (1970).
Yan, Y., Demerec, M.: Genetic analysis of pyrimidine mutants of Salmonella typhimurium. Genetics 52, 643–651 (1965).
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Communicated by W. Maas
Paper No. 1 of this series: Beck, C. F. and J. L. Ingraham, Molec. Gen. Genetics 111, 303–316 (1971).
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Beck, C.F., Ingraham, J.L. & Neuhard, J. Location on the chromosome of Salmonella typhinurium of genes governing pyrimidine metabolism. Molec. Gen. Genet. 115, 208–215 (1972). https://doi.org/10.1007/BF00268884
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DOI: https://doi.org/10.1007/BF00268884