Abstract
OmpH is an outer membrane protein produced by the deep-sea bacterium Photobacterium species strain SS9 in response to elevated hydrostatic pressure. In order to facilitate studies of the function of this protein, a series of OmpH+ and OmpH- strains were obtained from SS9 by Tn5 gene replacement mutagenesis. A previously isolated ompH::lacZ strain and a derivative of this strain harboring a plasmid expressing the wild-type ompH gene were also utilized. The acridine mutagen ICR 191 preferentially inhibited the growth of OmpH+ over OmpH- cells. Indeed, OmpH+ cultures treated with the mutagen rapidly accumulated mutants producing reduced levels of OmpH. In addition. OmpH+ cells took up the peptide Met-Leu-Phe approximately 15 times more rapidly than OmpH- cells. The results are consistent with the hypothesis that OmpH functions as a relatively large, nonspecific diffusion channel.
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Abbreviations
- OMP :
-
Outer membrane protein
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Bartlett, D., Chi, E. Genetic characterization of ompH mutants in the deep-sea bacterium Photobacterium sp. strain SS9. Arch. Microbiol. 162, 323–328 (1994). https://doi.org/10.1007/BF00263779
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DOI: https://doi.org/10.1007/BF00263779