Urological Research

, Volume 17, Issue 5, pp 299–303

Analysis of mucosal bladder leucocyte subpopulations in patients treated with intravesical Bacillus Calmette-Guerin

  • M. Peuchmaur
  • G. Benoit
  • A. Vieillefond
  • A. Chevalier
  • G. Lemaigre
  • E. D. Martin
  • A. Jardin
Original Articles

DOI: 10.1007/BF00262987

Cite this article as:
Peuchmaur, M., Benoit, G., Vieillefond, A. et al. Urol. Res. (1989) 17: 299. doi:10.1007/BF00262987

Summary

Immunohistochemical techniques were used to investigate leucocyte subpopulations in the bladders of patients with superficial transitional cell carcinoma treated with BCG Pasteur. Leucocyte subsets were enumerated with a panel of monoclonal antibodies which included CD3, CD4, CD8, TQ1, Leu7, CD15, HLA-DR, CD25, CD22. We demonstrated in the bladders of patients treated with BCG a particular lymphocyte population; the major subset was an inducer (CD4+, TQ1-) which was activated (CD25+, HLA-DR+) and associated with polymorphonuclear eosinophils. There was neither inducer of suppression nor major cytotoxic/suppressive subsets. CD8+ and NK cells could not be the primary mediators of BCG activity. These data supported the hypothesis of a helper T lymphocyte activity associated with lymphokine production and activation of effector killer cells.

Key words

Carcinoma of the bladderBCG therapyImmunohistochemical studiesLeucocyte subpopulationsHelper T lymphocyte

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Peuchmaur
    • 1
  • G. Benoit
    • 2
  • A. Vieillefond
    • 3
  • A. Chevalier
    • 1
  • G. Lemaigre
    • 1
  • E. D. Martin
    • 3
  • A. Jardin
    • 2
  1. 1.Department of PathologyAntoine Béclère HospitalClamart
  2. 2.Department of UrologyBicêtre HospitalLe Kremlin BicêtreFrance
  3. 3.Department of PathologyBicêtre HospitalLe Kremlin BicêtreFrance