Summary
Transgenic rice plants were obtained from protoplasts treated with two plasmids by electroporation. Primary transformants were selected on the basis of resistance to hygromycin, conferred by one of the co-transferred plasmids. Out of 26 hygromycin-resistant plants 2 showed the reporter gene activity due to another plasmid possessing a chimeric gene consisting of the promoter (about 900 by upstream non-coding region) of the ORF12 gene (roIC of the Ri plasmid and the coding region for β-glucuronidase (GUS). Using a colorimetric reaction, the GUS enzyme was found to be localized in vascular tissues, demonstrating the similar expression of the roIC gene promoter in monocots and dicots (Sugaya et al. 1989; Schmülling et al. 1989).
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Matsuki, R., Onodera, H., Yamauchi, T. et al. Tissue-specific expression of the rolC promoter of the Ri plasmid in transgenic rice plants. Molec. Gen. Genet. 220, 12–16 (1989). https://doi.org/10.1007/BF00260849
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DOI: https://doi.org/10.1007/BF00260849