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External factors involved in the regulation of an extracellular proteinase synthesis in Bacillus megaterium

The effect of glucose and amino acids

  • Applied Microbiology
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Summary

A mathematical model was formulated to describe the kinetics and stoichiometry of growth and proteinase production in Bacillus megaterium. Synthesis of the extracellular proteinase in a batch culture is repressed by amino acids. The specific rate of formation of the enzyme (r E) can be described by the formula {ie373-1}, where k 2 and k 3 stand for the non-repressible and repressible part of enzyme synthesis respectively, k S 2 is a repression coefficient and S 2 indicates the concentration of amono acids; the values of k 2 and k S 2 depend on the composition of the mixture of amino acids. Even in a high concentration, a single amino acid is less effective than a mixture of amino acids. The dependence of the proteinase repression on the concentration of an external amino acid (leucine) follows the same course as its rate of incorporation into proteins, approaching saturation at concentrations higher than 50 μM (half saturation approximately 10 μM). However, the total uptake of leucine did not exhibit any saturation even at 500 μM external concentration.

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Abbreviations

X :

biomass concentration, g/l

E :

proteinase concentration, unit/l

t :

time, h

S 1 :

concentration of glucose, g/l

S 2 :

concentration of amino acids, g/l

μ:

specific growth rate, l/h

rE :

specific rate of enzyme production, unit/g/h

k 1 :

growth kinetic constant, l/h

k 2 :

product formation kinetic constant (for non-repressible part of enzyme synthesis), unit/g

k 3 :

product formation kinetic constant (for repressible portion of enzyme synthesis), unit/g

k S 1 :

saturation constant, g/l

k S 2 :

repression coefficient for a certain amino acid or amino acids mixture, g/l

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Authors and Affiliations

  1. Department of Process Engineering and Computer Control, Czechoslovak Academy of Sciences, Vídeňská 1083, CS-142 20, Praha 4, Czechoslovakia

    Jaroslav Votruba

  2. Department of Enzyme Engineering of the Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeňská 1083, CS-142 20, Praha 4, Czechoslovakia

    Jarmila Pazlarová, Milada Dvořáková, Libuše Váchová, Marie Strnadová, Helena Kučerová & Jiří Chaloupka

  3. Institute of Chemical and Biological Physics, Academy of Sciences of Estonian Soviet Socialist Republic, Tallin, USSR

    Kalju Vanatalu

Authors
  1. Jaroslav Votruba
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  2. Jarmila Pazlarová
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  3. Milada Dvořáková
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  4. Kalju Vanatalu
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  5. Libuše Váchová
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  6. Marie Strnadová
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  7. Helena Kučerová
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  8. Jiří Chaloupka
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Votruba, J., Pazlarová, J., Dvořáková, M. et al. External factors involved in the regulation of an extracellular proteinase synthesis in Bacillus megaterium . Appl Microbiol Biotechnol 26, 373–377 (1987). https://doi.org/10.1007/BF00256673

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  • DOI: https://doi.org/10.1007/BF00256673

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