Theoretical and Applied Genetics

, Volume 84, Issue 3, pp 397–402

Characterization of species-specific repeated DNA sequences from B. nigra


  • Vibha Gupta
    • Tata Energy Research Institute
  • G. LakshmiSita
    • Microbiology and Cell Biology DepartmentIndian Institute of Science
  • M. S. Shaila
    • Microbiology and Cell Biology DepartmentIndian Institute of Science
  • V. Jagannathan
    • Tata Energy Research Institute
  • Malathi S. Lakshmikumaran
    • Tata Energy Research Institute

DOI: 10.1007/BF00229499

Cite this article as:
Gupta, V., LakshmiSita, G., Shaila, M.S. et al. Theoret. Appl. Genetics (1992) 84: 397. doi:10.1007/BF00229499


The construction and characterization of two genome-specific recombinant DNA clones from B. nigra are described. Southern analysis showed that the two clones belong to a dispersed repeat family. They differ from each other in their length, distribution and sequence, though the average GC content is nearly the same (45%). These B genome-specific repeats have been used to analyse the phylogenetic relationships between cultivated and wild species of the family Brassicaceae.

Key words

Repeated DNABrassica speciesGenome specificityPhylogenetic studies

Copyright information

© Springer-Verlag 1992