Theoretical and Applied Genetics

, Volume 92, Issue 3, pp 326–333

Abundance and length polymorphism of microsatellite repeats in Beta vulgaris L.

Authors

  • M. Mörchen
    • Laboratoire de Génétique et Evolution des Populations Végétales, URA-CNRS 1185, Bât. SN2Université de Lille 1
  • J. Cuguen
    • Laboratoire de Génétique et Evolution des Populations Végétales, URA-CNRS 1185, Bât. SN2Université de Lille 1
    • Institut Agricole et Alimentaire de LilleUniversité de Lille 1
  • G. Michaelis
    • Botanisches Institut der Heinrich-Heine Universität DüsseldorfUniversitätsstrasse 1
  • C. Hänni
    • Unité d'Oncologie Moléculaire, URA-CNRS 1160, Institut Pasteur de Lille
  • P. Saumitou-Laprade
    • Laboratoire de Génétique et Evolution des Populations Végétales, URA-CNRS 1185, Bât. SN2Université de Lille 1
Article

DOI: 10.1007/BF00223675

Cite this article as:
Mörchen, M., Cuguen, J., Michaelis, G. et al. Theoret. Appl. Genetics (1996) 92: 326. doi:10.1007/BF00223675

Abstract

Simple sequence repeats (SSRs) are known to exhibit high degrees of variability even among closely related individuals. Their usage as nuclear genetic markers requires their conversion into sequence-tagged sites (STSs). In this paper we present the development of simple sequences as STSs for Beta vulgaris. This species comprises wild, cultivated, and weedy forms; the latter are thought to originate from accidental hybridisation between the other two. Two partial genomic libraries were screened with simple sequence motifs (AT, CA, CT, ATT, GTG, and CA, CT, respectively). Clones of 22 CA, nine CT, eight ATT, and one GTG sequence were obtained. AT micro satellites were present in compound motifs, not recognised by the probe. Sequence comparisons revealed that 20 CA clones containing short motifs (<16 bp) were variants of a previously described approximately 320-bp satellite DNA (Schmidt et al. 1991), and hence did not correspond to unique loci. Polymorphism of one (ATT)15 and three (CT)n, with n=15, 17 and 26, was detected by PCR on a sample of 64 plants from the different forms of B. vulgaris. 13 (ATT), 13 (CT), nine (CT) alleles and one (CT) allele were detected. One of the ATT alleles was much larger than the others (>800 bp). Genetic variability was high among wild beets, lower among cultivated beets, and intermediate among weed beets. One allele of each locus was found at high frequencies in cultivated beets and, to a lower extent, in weed beets. The combination of three polymorphic loci allowed the individual identification of 17/17 wild and 15/15 weed beets, and 21/32, mostly homozygous, cultivated beets.

Key words

Beet VNTR Simple sequence length polymorphism Microsatellite Sequence-tagged sites

Copyright information

© Springer-Verlag 1996