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Co-immobilization of amyloglucosidase and pullulanase for enhanced starch hydrolysis

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Summary

Amyloglucosidase and pullulanase were co-immobilized using a hydrophilic polyurethane foam (Hypol® 2002). The combined amyloglucosidase and pullulanase activity of the immobilized enzyme was 32.2% ± 1.7% relative to the non-immobilized enzyme. The co-immobilized enzymes were capable of using a variety of glycogen and starch substrates. Co-immobilization of amyloglucosidase and pullulanase increased the glucose yield 1.6-fold over immobilized amyloglucosidase alone. No decrease in activity was observed after 4 months storage for the co-immobilized enzymes. The results suggest that co-immobilization of amyloglucosidase and pullulanase in polyurethane foams is a potentially useful approach for commercial starch hydrolysis.

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References

  • Chakrabarti AC, Storey KB (1988) Immobilization of cellulase using polyurethane foam. Appl Biochem Biotechnol 19:189–207

    Google Scholar 

  • Chakrabarti AC, Storey KB (1990) One-step conversion of cellulose to fructose using co-immobilized cellulase, β-glucosidase and glucose isomerase. Appl Biochem Biotechnol, in press

  • Cheetham PSJ (1985) Principles of industrial enzymology: basis of utilization of soluble and immobilized enzymes in industrial processes. In: Wiseman A (ed) Handbook of enzyme biotechnology, 2nd ed. Ellis Horwood, Rexdale, pp 157–181

    Google Scholar 

  • Galliard T (1985) Bulk chemicals from plants: starch and starch-derived products. Ann Proc Phytochem Soc Eur 26:103–115

    Google Scholar 

  • Lowry OH, Passonneau JV (1972) A flexible system of enzymatic analysis. Academic Press, New York, pp 174–177

    Google Scholar 

  • Storey KB, Duncan JA, Chakrabarti AC (1990) Immobilization of amyloglucosidase using two forms of polyurethane polymer. Appl Biochem Biotechnol, in press

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Chakrabarti, A.C., Storey, K.B. Co-immobilization of amyloglucosidase and pullulanase for enhanced starch hydrolysis. Appl Microbiol Biotechnol 33, 48–50 (1990). https://doi.org/10.1007/BF00170568

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  • DOI: https://doi.org/10.1007/BF00170568

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