Biotechnology Techniques

, Volume 9, Issue 12, pp 897–900

Isolation of virally-infected insect cells from a population containing infected and uninfected cells

  • Alexander Matschiner
  • Jonathan S. Dordick
  • David W. Murhammer
Article

DOI: 10.1007/BF00158543

Cite this article as:
Matschiner, A., Dordick, J.S. & Murhammer, D.W. Biotechnol Tech (1995) 9: 897. doi:10.1007/BF00158543

Abstract

A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Specifically, Spodoptera frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Alexander Matschiner
    • 1
  • Jonathan S. Dordick
    • 1
  • David W. Murhammer
    • 1
  1. 1.Department of Chemical and Biochemical EngineeringThe University of IowaIowa CityUSA