Abstract
The effects of human recombinant interleukin-1α and β (rIL-1α; rIL-1β) on the adhesion of human A549 lung carcinoma cells and M6 melanoma cells (TC) to human endothelial cells (HECs) in vitro were studied, and on TC/lung entrapment in vivo. In vitro, there was a significant increase in TC/HEC adhesion to HECs pretreated for 4 h with rIL-1α or rIL-1β. The effects of rIL-1α and β on TC/HEC adhesion were time dependent and reached a plateau within 4–6h. TC/HEC adhesion was not blocked when measured in the presence of antibodies to either fibronectin, glycoprotein IIb/IIIa, anti-ICAM, or anti-LFA. However, enhanced TC/HEC adhesion was completely blocked in the presence of the peptide, GRGDS. In vivo, pretreatment of nude mice for 4 h with rIL-1α (given i.p. before ix. injection of TCs) enhanced TC retention in the lung 24 h later. Our data demonstrate that IL-1 enhances TC adhesion to the vascular surface both in vitro and in vivo, suggesting that IL-1 can facilitate the metastatic process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Auerbach, R., Wei Cheng, L., Pardon, E., Gumkowski, F., Kaminska, G., and Kaminski, M., 1987,Specificity of adhesion between tumor cells and capillary endothelium: An in vitro correlate of preferential metastasis in vivo. Cancer Research, 47, 1492–1496.
Bastida, E., Almirall, L., Buchanan, M. R., Haas, T. A., Lauri, D., and Orr, W., 1987, Effects of 13-HODE and HETEs on tumor cell/endothelial cell interactions. Thrombosis and Haemostasis, 58, 315.
Bevilaqua, M. P., Pober, J. S., Mendrick, D. L., Cotran, R. S., and Gimbrone, M. A. Jr, 1987, Identification of an inducible endothelial-leukocyte adhesion molecule, ELAM-1. Proceedings of the National Academy of Sciences, 84 (8), 9238–9242.
Bevilacqua, M. P., Pober, J. S., Wheeler, M. E., Cotran, R. S., and Gimbrone, M. A. Jr, 1985, Interleukin-1 acts on cultured human vascular and endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes and related leukocyte cell lines. Journal of 10585ical Investigation, 76, 2003–2011.
Buchanan, M. R., and Bastida, E., 1987, The role of 13-HODE and HETEs in vessel wall/ circulating blood cell interaction. Agents and Actions, 22(3/4), 337–339.
Lavender, D. E., Haskard, D. O., Joseph, B., and Ziff, M., 1986, Interleukin-1 increases the binding of human B and T lymphocytes to endothelial cell monolayers. Journal of Immunology, 136, 203–207.
Haas, T., Bastida, E., Nakamura, K., Hullin, J., Almirall, L., and Buchanan, M. R., 1988, Binding of 13-HODE 5-, 12- and 15-HETE to endothelial cells and subsequent platelet, neutrophil and tumor cell adhesion. Biochimica et Biophysica Acta, 961, 153–159.
Humphries, M. J., Olden, K., and Yamada, K. M., 1986, A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells. Science, 233, 467–469.
Humphries, M. J., Yamada, K. M., and Olden, K., 1988, Investigation of the biological effects of an anti-cell adhesive synthetic peptide that inhibit experimental metastasis of B16 F10 murine melanoma cells. Journal of 10585ical Investigation, 81, 782–790.
Jaffe, E. A., Nachman, R. L., Becker, D. G., and Minick, C. R., 1973, Culture of human endothelial cells derived from the umbilical veins. Journal of 10585ical Investigation, 52, 2745–2756.
Kramer, R. H., Gonzalez, R., and Nicolson, G. L., 1980, Metastatic tumor cells adhere preferentially to the extracellular matrix underlying vascular endothelial cells. International Journal of Cancer, 26, 639–645.
Lauri, D., Bertomeu, M. C., Bastida, E., Orr, F. W., and Buchanan, M. R., 1988, Interleukin-1 increases tumor cell adhesion to endothelial cells: In vitro and in vivo studies. Proceedings of the International Symposium on the Vascular Cell, Book of Abstracts, July 16–30, Toronto, Canada, P-III-7.
Liao, S. K., Dent, P. B., and MCCulloch, P. B., 1975, Characterization of human malignant melanoma cell lines. Morphology and growth characteristics in culture. Journal of the National Cancer Institute, 54, 1037–1044.
Liotta, L. A., 1984, Tumor invasion and metastases: Role of the basement membrane. Warner Lambert Parke-Davis Award Lecture. American Journal of Pathology, 117, 339–348.
Orr, F. W., Adamson, I. Y. R., and Young, L., 1986, Promotion of pulmonary metastases in mice by bleomycin induced endothelial injury. Cancer Research, 46, 891–897.
Plow, E. F., LOftus, J. C., Levin, E. G., Fair, D. S., Dixon, D., Forsyth, J., and Ginsberg, M. H., 1983, Immunological relationship between platelet membrane glycoprotein GP IIb/IIIa on cell surface molecules expressed by a variety of cells. Proceedings of the National Academy of Sciences, 83, 6002–6007.
Rothlein, R., Dustin, M. L., Marlin, S. D., and Springer, T. A., 1986, A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. Journal of Immunology, 137, 1270–1274.
Ruoslahti, E., and Pierschbacher, M. D., 1986, Arg-Gly-Asp: versatile cell recognition signal. Cell, 44, 517–518.
Vilella, R., Lozano, T., Milla, J., Borche, L., Ercilla, G., Ordinas, T., and Vives, J., 1984, An antiplatelet monoclonal antibody that inhibits ADP and epinephrine-induced aggregation. Thrombosis and Haemostasis, 51, 93–96.
Weiss, L., 1985, Principle of metastasis (New York: Academic Press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lauri, D., Bertomeu, MC., Orr, F.W. et al. Interleukin-1 increases tumor cell adhesion to endothelial cells through an RGD dependent mechanism: in vitro and in vivo studies. 10585 Exp Metast 8, 27–32 (1990). https://doi.org/10.1007/BF00155590
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00155590