Article

Biotechnology Letters

, Volume 18, Issue 3, pp 235-240

Production of biosurfactants by Cladosporium resinae

  • Joaquín M. MurielAffiliated withDepartamento de Bioquímica y Biología Molecular y Genética, Universidad de Extremadura
  • , José M. BruqueAffiliated withDepartamento de Física Aplicada, Facultad de Ciencias, Universidad de Extremadura
  • , José Manuel OlíasAffiliated withInstituto de la Grasa, C.S.I.C.
  • , Alfonso Jiménez-SánchezAffiliated withDepartamento de Bioquímica y Biología Molecular y Genética, Universidad de Extremadura

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Summary

Cladosporium resinae produces extracellular biosurfactants when growing in a hydrocarbon source such as the jet fuel JP8. This production of biosurfactants was observed by the reduction of the surface tension of the aqueous phase of growing medium, and by the increase in emulsion and foaming properties. A partial purification by collapsed foam gave better physical properties by decreasing surface tension and increasing foaming power and stabilization of emulsions. Surface active substances were purified by reversed phase chromatography. Six compounds representing over 75% of fraction containing surface activity were present. This fraction gave an improvement of all surface properties.