Summary
To characterize the molecular properties of CGTase from alkalophilic Bacillus sp. E1 (BCGTE1), a genomic clone for a CGTase was isolated. Expression of recombinant BCGTE1 in E. coli was analyzed by immunoblotting. It showed that the nascent recombinant BCGTE1 expressed was 87 kDa but it was processed into the mature enzyme of 81 kDa. With the process it was secreted predominantly into the culture medium via periplasmic space. This feature is different from other Bacillus CGTases expressed in E. coli, which were present mostly in the periplasmic space.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Binder, F., Huber, O. and Bock, A. (1986). Gene. 47, 269–277
Burnette, W. N. (1981). Anal. Biochem. 112, 195–203
Dubnau, D. and Abenson, R. D. (1971). J. Mol. Biol. 56, 209–221
Fujiwara, S., Kakihara, H., Kim, B. W., Lejeune, A., Kanemoto, M., Sakaguchi, K. and Imanaka, T. (1992a). Appl. Environ. Microbiol. 58, 4016–4025
Fujiwara, S., Kakihara, H., Sakaguchi, K. and Imanaka, T. (1992b). J. Bacteriol. 174, 7478–7481
Gunnar von Heijne (1986). Nucleic Acids Res. 14, 4383–4690
Itkor, P., Tsukagoshi, N. and Udaka, S. (1990). Biochem. Biophys. Res. Commun. 166, 630–636
Kaneko, T., Hamamoto, T. and Horikoshi, K. (1988). J. Gen. Microbiol. 134, 97–105
Kimura, K., Kataoka, S., Nakamura, A., Takano, T., Kobayashi, S. and Yamane, K. (1989). Biochem. Biophys. Res. Commun. 161, 1273–1279
Kitamoto, N., Kimura, K., Kito, Y. and Ohmiya, K. (1992). J. Fermen. Bioeng. 74, 345–351
Lee, K. C. P and Tao, B. Y. (1994). Starch. 46, 67–74
Nitschke, L., Heeger, K., Bender, H. and Schulz, G. E. (1990). Appl. Microbiol. Biotechnol. 33:542–546
Park, C. S., Woo, E. J., Kuk, S. U., Seo, B. C., Park, K. H. and Lim, H. (1992). Kor. J. Microbiol. Biotechnol. 20, 156–163
Paloheimo, M., Haglund, D., Aho, S. and Korhola, M. (1992). Appl. Microbiol. Biotechnol. 36, 584–591
Pugsley, A. P. (1993). Microbiol. Rev. 57, 50–108
Sambrook, J, Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sin, K. A., Nakamura, A., Kobayashi, K., Masaki, H. and Uozumi, T. (1991). Appl. Microbiol. Biotechnol. 35, 600–605
Ueda, H. and Nagai, T. (1988). In: Abstracts of 4th Int. Symp. on Cyclodextrins. Munich, FRG., p23
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yong, J., Choi, J.N., Park, S.S. et al. Secretion of heterologous cyclodextrin glycosyltransferase of Bacillus sp. E1 from Escherichia coli . Biotechnol Lett 18, 1223–1228 (1996). https://doi.org/10.1007/BF00128597
Issue Date:
DOI: https://doi.org/10.1007/BF00128597