TS/A: a new metastasizing cell line from a BALB/c spontaneous mammary adenocarcinoma
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
A metastasizing mouse cell line (TS/A), originated from a mammary adenocarcinoma which arose spontaneously in a BALB/c female retired breeder, has been established in vitro. It displayed a remarkable morphologic heterogeneity, which is evident in plastic adherent cultures (cell types ranging from epithelial-like to fibroblast-like) as well as in semi-solid agar cultures. The TS/A line exhibited the presence of specific cytoplasmic estradiol receptor, with a binding activity of 16 fmoles/mg cytosol protein. The in vivo growth pattern was as follows: (1) a s.c. inoculum of 105 cells caused a 100 per cent tumor take and kill in syngeneic animals; mean survival time was 54 + 1 days; (2) it did not show significant transplant immunogenicity in syngeneic animals; (3) it was able to give rise to both spontaneous lung metastases and artificial lung colonies; (4) it had a high capacity to grow in H-2 matched, minor histocompatibility antigen incompatible hosts (106 cells killed 100 per cent DBA/2 mice in 58 + 2 days). This line of spontaneous mammary tumor cells is proposed as a useful model for studies on the heterogeneity of the neoplastic population in relation to metastatic spread, on tumor immunogenicity, and on therapy of mammary neoplasia.
- Alexander, P., 1982, The role of the host in metastasis. Accomplishments in Cancer Research 1981, edited by J. G. Fortner and J. E. Rhoads (Philadelphia-Toronto: J. B. Lippincott Company), pp. 138–148.
- Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248–254.
- Chandradasa, K. D., and Blears, J., 1982, Studies with a spontaneous murine tumour—I. Indetectability of host immune resistance with tumour-activated elaboration of T cell stimulatory lymphokines in vitro. European Journal of Cancer and Clinical Oncology, 18, 853–859.
- Chen, T. R., 1977, In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Experimental Cell Research, 104, 255–262.
- Danielson, K. G., Anderson, L. W., and Hosick, H. L., 1980, Selection and characterization in culture of mammary tumor cells with distinctive growth properties in vivo. Cancer Research, 40, 1812–1819.
- Dexter, D. L., Kowalski, H. M., Blazar, B. A., Fligiel, Z., Vogel, R., and Heppner, G., 1978, Heterogeneity of tumor cells from a single mouse mammary tumor. Cancer Research, 38, 3174–3181.
- Fidler, I. J., and Kripke, M. L., 1977, Metastasis results from preexisting variant cells within a malignant tumor. Science, 197, 893–895.
- Forni, G., Varesio, L., Giovarelli, M., and Landolfo, S., 1979, Source of tumor cells influences their immune recognition by H-2-matched mice. Transplantation, 27, 433–435.
- Fumero, S., Berruto, G. P., Pelizzola, D., Grilli, S., Buttazzi, C., Di Fronzo, G., Ronchi, E., Bozzetti, C., Mort, P., Concolino, G., Marocchi, A., Robustelli, Della Cuna, G., Zibera, C., Cerrutti, G., Ros, A., and Piffanelli, A., 1981, Results of the Italian Interlaboratory quality control program for estradiol receptor assay. Tumori, 67, 301–306.
- Galili, N., Naor, B., Åsiö, B., and Klein, G., 1976, Induction of immune responsiveness in a genetically low-responsive tumor-host combination by chemical modification of the immunogen. European Journal of Immunology, 6, 473–476.
- Hewitt, H. B., Blake, E. R., and Walder, A. S., 1976, A critique of the evidence for host defense against cancer based on personal studies of 27 murine tumours of spontaneous origin. British Journal of Cancer, 33, 241–259.
- Hewitt, H. B., 1982, Animal tumor models and their relevance to human tumor immunology. Journal of Biological Response Modifiers, 1, 107–119.
- Nanni, P., De Giovanni, C., Galli, M. C., Grilli, S., Lollini, P.-L., Nicoletti, G., and Prodi, G., 1981, Minor histocompatibility antigens do not enhance BALB/c anti-SV40 TASA response. British Journal of Cancer, 44, 588–591.
- Wexler, H., 1966, Accurate identification of experimental pulmonary metastases. Journal of National Cancer Institute, 36, 641–645.
- TS/A: a new metastasizing cell line from a BALB/c spontaneous mammary adenocarcinoma
Clinical & Experimental Metastasis
Volume 1, Issue 4 , pp 373-380
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Industry Sectors