Clinical & Experimental Metastasis

, Volume 1, Issue 4, pp 373–380

TS/A: a new metastasizing cell line from a BALB/c spontaneous mammary adenocarcinoma


  • Patrizia Nanni
    • Istituto di CancerologiaUniversità di Bologna
  • Carla De Giovanni
    • Istituto di CancerologiaUniversità di Bologna
  • Pier-Luigi Lollini
    • Istituto di CancerologiaUniversità di Bologna
  • Giordano Nicoletti
    • Istituto di CancerologiaUniversità di Bologna
  • Giorgio Prodi
    • Istituto di CancerologiaUniversità di Bologna

DOI: 10.1007/BF00121199

Cite this article as:
Nanni, P., De Giovanni, C., Lollini, P. et al. Clin Exp Metast (1983) 1: 373. doi:10.1007/BF00121199

A metastasizing mouse cell line (TS/A), originated from a mammary adenocarcinoma which arose spontaneously in a BALB/c female retired breeder, has been established in vitro. It displayed a remarkable morphologic heterogeneity, which is evident in plastic adherent cultures (cell types ranging from epithelial-like to fibroblast-like) as well as in semi-solid agar cultures. The TS/A line exhibited the presence of specific cytoplasmic estradiol receptor, with a binding activity of 16 fmoles/mg cytosol protein. The in vivo growth pattern was as follows: (1) a s.c. inoculum of 105 cells caused a 100 per cent tumor take and kill in syngeneic animals; mean survival time was 54 + 1 days; (2) it did not show significant transplant immunogenicity in syngeneic animals; (3) it was able to give rise to both spontaneous lung metastases and artificial lung colonies; (4) it had a high capacity to grow in H-2 matched, minor histocompatibility antigen incompatible hosts (106 cells killed 100 per cent DBA/2 mice in 58 + 2 days). This line of spontaneous mammary tumor cells is proposed as a useful model for studies on the heterogeneity of the neoplastic population in relation to metastatic spread, on tumor immunogenicity, and on therapy of mammary neoplasia.

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© Taylor & Francis Ltd 1983