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Physiology of microbial degradation of chitin and chitosan

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Abstract

Chitin is produced in enormous quantities in the biosphere, chiefly as the major structural component of most fungi and invertebrates. Its degradation is chiefly by bacteria and fungi, by chitinolysis via chitinases, but also via deacetylation to chitosan, which is hydrolysed by chitosanases. Chitinases and chitosanases have a range of roles in the organisms producing them: autolytic, morphogenetic or nutritional. There are increasing examples of their roles in pathogenesis and symbiosis. A range of chitinase genes have been cloned, and the potential use for genetically manipulated organisms over-producing chitinases is being investigated. Chitinases also have a range of uses in processing chitinous material and producing defined oligosaccharides.

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References

  • Aruchami, M, Sundara-Rajulu, G & Gowri, N (1986) Distribution of deacetylase in arthropoda. In: Muzzarelli, R, Jeuniaux, C & Gooday, GW (Eds) Chitin in Nature and Technology (pp 263–265). Plenum Press, New York

    Google Scholar 

  • Baross, JA, Tester, PA & Morita, RY (1978) Incidence, microscopy and etiology of exoskeleton lesions in the tanner crab Chionectes tanner. J Fish Res Board Can 35: 1141–1149

    Google Scholar 

  • Barrett-Bee, K & Hamilton, M (1984) The detection and analysis of chitinase activity from the yeast form of Candida albicans. J Gen Microbiol 130: 1857–1861

    Google Scholar 

  • Bartnicki-Garcia, S (1973) Fundamental aspects of hyphal morphogenesis. In: Ashworth, JM & Smith, JE (Eds) Microbial Differentiation (pp 245–268). Cambridge University Press, Cambridge

    Google Scholar 

  • Bartnicki-Garcia, S & Lippman, E (1982) Fungal wall composition. In Laskin, AJ & Lechevalier, HA (Eds) CRC Handbook of Microbiology, 2nd Ed. Vol. IV, Microbial Composition: Carbohydrates, Lipids and Minerals (pp 229–252). CRC Press, Boca Raton

    Google Scholar 

  • Bassler, B., Gibbons, P & Roseman, S (1989) Chemotaxis to chitin oligosaccharides by Vibrio furnissii, a chitinivorous marine bacterium. Biochem Biophys Res Comm 161: 1172–1176

    Google Scholar 

  • Benhamou, N & Asselin, A (1989) Attempted localization of a substrate for chitinases in plant cells reveals abundant N-acetyl-D-glucosamine residue in secondary walls. Biol Cell 67: 341–250

    Google Scholar 

  • Berkeley, RCW (1979) Chitin, chitosan and their degradative enzymes. In: Berkeley, RCW, Gooday, GW & Ellwood, DC (Eds) Microbial Polysaccharides and Polysaccharases (pp 205–236). Academic Press, London

    Google Scholar 

  • Beyer, M & Diekmann, H (1985) The chitinase system in Streptomyces sp. ATCC 11238 and its significance for fungal cell wall degradation. Appl Microbiol Biotech 23: 14–146

    Google Scholar 

  • Bibochka, MJ & Khachatourians (1988) N-Acetyl-D glucosamine-mediated regulation of extracellular protease in the entomopathogenic fungus Beauvaris bassiana. Appl Environ Microbiol 54: 2699–2704

    Google Scholar 

  • Blackwell, J (1988) Physical methods for the determination of chitin structure and conformation. Meth Enzymol 161: 435–442

    Google Scholar 

  • Borkott, H & Insam, H (1990) Symbiosis with bacteria enhances the use of chitin by the springtail, Folsomia candida (Collembola). Biol Fert Soils 9: 126–129

    Google Scholar 

  • Bradbury, P, Deroux, G & Campillo, A (1987) The feeding of a chitinivorous ciliate. Tissue Cell 19: 351–363

    Google Scholar 

  • Broglie, KE, Gaynor, JJ Broglie, RM (1986) Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proc Nat Acad Sci USA 86: 6820–6824

    Google Scholar 

  • Cabib, E, Silverman, SJ, Sburlati, A & Slater, ML (1990) Chitin synthesis in yeast Saccharomyces cerevisiae. In: Kuhn, PJ, Trinci, APJ, Jung, MJ, Goosey, MW & Copping, LG (Eds) Biochemistry of Cell Walls and Membranes in Fungi (pp 31–41). Springer-Verlag, Berlin

    Google Scholar 

  • Chan J G (1970) The Occurrence, Taxonomy and Activity of Chitinolytic Bacteria from Sediment, Water and Fauna of Puget Sound. Ph.D. thesis, University of Washington, Seattle

  • Dackman, C, Chet, I & Nordbring-Hertz, B (1989) Fungal parasitism of the cyst nematode Heterodera schachtii: infection and enzymatic activity. FEMS Microbiol Ecol 62 201–208

    Google Scholar 

  • Daoust, RA & Gunner, HB (1979) Microbial synergists pathogenic to Lymantria dispar: chitinolytic and fermentative bacterial interactions. J Invert Path 33: 368–377

    Google Scholar 

  • Datema, R, Ende, Hvan den & Wessels, JGH (1977) The hyphal wall of Mucor mucedo 2. Hexosamine-containing polymers. Eur J Biochem. 80: 621–626

    Google Scholar 

  • Davis, B & Eveleigh, DE (1984) Chitosanases: occurrence, production and immobilization. In: Zikakis, JP (Ed) Chitin, Chitosan and Related Enzymes (pp 161–179). Academic Press, Orlando

    Google Scholar 

  • Davis, LL & Bartnicki-Garcia, S (1984) The co-ordination of chitosan and chitin synthesis in Mucor rouxii. J Gen Microbiol 130: 2095–2102

    Google Scholar 

  • Derksen, ACG & Granados, RR (1988) Alteration of a lepidoteran peritrophic membrane by baculoviruses and enhancement of viral infectivity. Virology 167: 242–250

    Google Scholar 

  • Dunsmuir, P & Suslow, T (1989) Structure and regulation of organ- and tissue-specific-genes: chitinase genes in plants. In: Schell, J & Vaszil, IK (Eds) Cell Culture and Somatic Cell Genetics in Plants, Vol 6, Molecular Biology of Plant Nuclear Genes (pp 215–227). Academic Press, San Diego

    Google Scholar 

  • Elango, N, Correa, JV, Cabib, E (1982) Secretory nature of yeast chitinase. J Biol Chem 257: 1398–1400

    Google Scholar 

  • Flyg, C & Boman, HG (1988) Drosphila genes cut and miniature are associated with the susceptibility to infection in Serratia marcescens. Genet Res 52: 51–56

    Google Scholar 

  • Fuchs, R, McPherson, S & Drahos, D (1986) Cloning of a Serratia marcescens gene encoding chitinase. Appl Environ Microbiol 51: 504–509

    Google Scholar 

  • Gooday, GW (1979) A survey of polysaccharase production: a search for phylogenetic implications. In: Berkeley, RCW, Gooday, GW & Ellwood, DC (Eds) Microbial Polysaccharides and Polysaccharaes (pp 437–460). Academic Press, London

    Google Scholar 

  • (1990a) The ecology of chitin degradation. In: Marshall, KC (Ed) Advances in Microbial Ecology, Vol. 11 (pp 387–430). Plenum Press, New York

  • (1990b) Inhibition of chitin metabolism. In: Kuhn, PJ, Trinci, APJ, Jung, MJ, Goosey, MW & Copping, LG (Eds) Biochemistry of Cell Walls and Membranes in Fungi (pp 61–79). Springer-Verlag, Berlin

  • (1990c) Chitinases. In: Leatham, G & Himmel, M (Eds) Enzymes in Biomass Conversion. American Chemical Society Books, Washington

  • (1990d) Chitin metabolism: a target for antifungal and antiparasitic drugs. Pharmacol Ther Suppl 175–185

  • Gooday, GW & Gow, NAR (1991) The enzymology of tip growth in fungi. In: Heath, IB (Ed) Tip Growth of Plant and Fungal Cells (pp 31–58). Academic Press, New York

    Google Scholar 

  • Gooday, GW, Humphreys, AM & McIntosh, WH (1986) Roles of chitinase in fungal growth. In: Muzzarelli, RAA, Jeuniaux, C & Gooday, GW (Eds) (pp 83–91). Plenum Press, New York

    Google Scholar 

  • Gooday GW, Prosser JI, Hillman K & Cross M (1991) Mineralization of chitin in an estuarine sediment: the importance of the chitosan pathway. Biochem Systematics Ecol (in press)

  • Gow, NAR & Gooday, GW (1983) Ultrastructure of chitin in hyphae of Candida albicans and other dimorphic and mycelial fungi. Protoplasma 115: 52–58

    Google Scholar 

  • Hara, S, Yamamura, Y, Fujii, Y, Mega, T & Ikenaka, T (1989) Purification and characterization of chitinase produced by Streptomyces erythraeus. J Biochem 105: 484–489

    Google Scholar 

  • Hedges, A & Wolfe, RS (1974) Extracellular enzyme from Myxobacter AL-1 that exhibits both β-1,4-glucanase and chitosanase activities. J Bacteriol 120: 844–853

    Google Scholar 

  • Hellebust, JA & Lewin, J (1977) Heterotrophic nutrition. In: Werner, D (Ed) The Biology of Diatoms (pp 169–197). Blackwell, Oxford

    Google Scholar 

  • Helmke, E & Weyland, H (1986) Effect of hydrostatic pressure and temperature on the activity and synthesis of chitinases of Antarctic Ocean bacteria. Mar Biol 91: 1–7

    Google Scholar 

  • Herwig, RP, Staley, JT, Nerini, MK & Braham, HW (1984) Baleen whales: preliminary evidence for forestomach microbial fermentation. Appl Environ Microbiol 47 421–423

    Google Scholar 

  • Hillman, K, Gooday, GW & Prosser, JI (1989a) The mineralization of chitin in the sediments of the Ythan Estuary, Aberdeenshire, Scotland. Estuarine Coastal Shelf Sci 29: 601–612

    Google Scholar 

  • (1989b) A simple model system for small scale in vitro study of estuarine sediment ecosystems. Lett Appl Microbiol 4: 41–44

    Google Scholar 

  • Hirano, S & Tokura, S (1982) Chitin and Chitosan. Japanese Society of Chitin and Chitosan. Tottori

    Google Scholar 

  • Hood, MA & Meyers, SP (1977) Microbial and chitinoclastic activities associated with Panaeus setiferus. J Oceangraph Soc Japan 33 235–241

    Google Scholar 

  • Horwitz, M, Reid, J & Ogaydziak, D (1984) Genetic improvements of chitinase production of Serratia marcescens. In: Zikakis, J (Ed) Chitin, Chitosan and Related Enzymes (pp 191–189). Academic Press, Orlando

    Google Scholar 

  • Humphreys, AM & Gooday, GW (1984a) Properties of chitinase activities from Mucor mucedo: Evidence for a membrane-bound zymogenic form. J Gen Microbiol 130: 1359–1366

    Google Scholar 

  • (1984b) Phospholipid requirement of microsomal chitinase from Mucor mucedo. Curr Microbiol 11: 187–190

    Google Scholar 

  • (1984c) Chitinase activities from Mucor mucedo. In: Nombela, C. (Ed) Microbial Cell Wall Synthesis and Autolysis (pp 269–273). Elsevier, Amsterdam

  • Isaac, S & Gokhale, AV (1982) Autolysis: a tool for protoplast production from Aspergillus nidulans. Trans Br Mycol Soc 78: 389–394

    Google Scholar 

  • Isogai, A, Sato, M, Sakuda, S, Nakuyama, A (1989) Structure of demethylallosamidin as an insect chitinase inhibitor. Agric Biol Chem 53: 2825–2826

    Google Scholar 

  • Iten, W & Matile, P (1970) Role of chitinase and other lysosomal enzymes of Coprinus lagopus in the autolysis of fruiting bodies. J Gen Microbiol 61: 301–309

    Google Scholar 

  • Iverson, KL, Bromel, MC, Anderson, Aw & Freeman, TP (1984) Bacterial symbionts in the sugar beet root maggot Tetanops myopaeformis (von Röder), Appl Environ Microbiol 47: 22–27

    Google Scholar 

  • Janszen, FH & Wessels, JGH (1970) Enzymic dissolution of hyphal septa in a Basidiomycete. Antonie van Leewenhoek 36: 255–257

    Google Scholar 

  • Jeuniaux, C (1963) Chitine and Chitinolyse. Masson, Paris

    Google Scholar 

  • (1982) La chitine dans le régne animal, Bull Soc Zool France 107: 363–386

    Google Scholar 

  • Jones, J, Grady, K, Suslow, T & Bedbrook, J (1986) Isolation and characterization of genes encoding two distinct chitinase enzymes from Serratia marcescens EMBO J 5: 467–473

    Google Scholar 

  • Jones, JDG, Dean, C, Gidoni, D, Gilbert, D, Bond-Nutter, D, Nedbrook, J & Dunsmuir, P (1988) Expression of a bacterial chitinase protein in tobacco leaves using two photosynthetic gene promoters. Mol Gen Genet 212: 536–542

    Google Scholar 

  • Kamei, K, Yamamura, Y, Hara, S & Ikenda, T (1989) Amino acid sequence of chitinase from Streptomyces erythaeus. J Biochem 105: 979–985

    Google Scholar 

  • Kaneko, T & Colwell, RR (1978) The annual cycle of Vibrio parahaemolyticus in Chesapeake Bay. Microbial Ecol 4: 135–155

    Google Scholar 

  • Kohlmeyer, J (1972) Marine fungi deteroriating chitin of hydrozoa and keratin-like annelid tubes. Mar Biol 12: 277–284

    Google Scholar 

  • Kole, MM & Altosaar, I (1985) Increased chitinase production by a non-pigmented mutant of Serratia marcescens. FEMS Microbial Lett 26: 265–269

    Google Scholar 

  • Kuhl, J, Nittinger, J & Siebert, G (1978) Vervetang von Krillschalen in Futterungsversuchen an de Ratte, Arch Fischereiwiss 29: 99–103

    Google Scholar 

  • Kuranda, MJ & Robbins, PW (1988) Cloning and heterologus expression of glycosidase genes from Saccharomyces cerevisiae. Proc Nat Acad Sci USA 84: 2585–2589

    Google Scholar 

  • Lindsay, GJH & Gooday, GW (1985a) Action of chitinase in spines of the diatom Thalassiosira fluviatilis. Carbohydr Polymers 5 131–140

    Google Scholar 

  • (1985b) Chitinolytic enzymes and the bacterial microflora in the digestive tract of cod, Gadus morhua. J Fish Biol 26: 255–265

    Google Scholar 

  • Lindsay, GJH, Walton, MJ, Adron, JW, Fletcher, TC, Cho, CY & Cowey, CB (1984) The growth of rainbow trout (Salmo gairdneri) given diets containing chitin and its relationships to chitinolytic enzymes and chitin digestibility. Aquaculture 37: 315–334

    Google Scholar 

  • Linthorst HJM, Loon LC van, Rossum CMA van, Mayer, A, Bol JF, Roekel JSC van, Meulenhoff EJS & Cornelissen BJC (1990) Analysis of acid and basic chitinases from tobacco and petunia and their constitutive expression in transgenic tobacco. Mol Plant-Microbe Interact (in press)

  • Lund, P, Lee, RY & Dunsmuir, P (1989) Bacterial chitinase is modified and secreted in transgenic tobacco. Plant Physiol 91: 130–135

    Google Scholar 

  • Lynch, J (1987) In vitro identification of Trichoderma harzianum as a potential antagonist of plant pathogens. Curr Microbiol 16: 49–53

    Google Scholar 

  • Lysenko, O (1976) Chitinase of Serratia marcescens and its toxicity to insects, J Invertebr Pathol 27: 385–386

    Google Scholar 

  • Mauch, F, Mauch-Mani, B & Boller, T (1988) Antifungal hydrolases in pea tissue. Inhibition of fungal growth by combination of chitinase and β-1,3-glucanase. Plant Physiol 87: 936–942

    Google Scholar 

  • Mauch, F & Staehelin, LA (1989) Functional implications of the subcellular localization of ethylene-induced chitinase and β-1,3-glucanase in bean leaves. Plant Cell 1: 447–457

    Google Scholar 

  • Maudlin, I & Welburn, SC (1988) Parasit Today 4: 109–111

    Google Scholar 

  • Monaghan RL (1975) The Discovery, Distribution and Utilization of Chitosanase. Ph.D. Thesis, Rutgers University, New Brunswick, N.J.

  • Monaghan, RL, Eveleigh, DE, Tewari, RP & Reese, ET (1973) Chitosanase, a novel enzyme, Nature (London) New Biol 245: 78–80

    Google Scholar 

  • Monreal, J & Reese, ET (1969) The chitinase of Serratia marcescens. Can J Microbiol 15: 689–696

    Google Scholar 

  • Murray, CL & Lovett, J S (1966) Nutritional requirements of the chytrid, Karlingia asterocysta, an obligate chitinophile. Am J Bot 53: 469–476

    Google Scholar 

  • Muzzarelli RAA & Pariser ER (1978) Proceedings of the First International Conference on Chitin/Chitosan. MIT Sea Grant Report MITSG

  • Muzzarelli, RAA, Jeuniaux, C & Gooday, GW (1986) Chitin in Nature and Technology. Plenum, New York

    Google Scholar 

  • Nanjo, F, Sakai, K, Ishikawa, M, Isobe, K & Usui, T (1989) Agric Biol Chem 53: 2189–2195

    Google Scholar 

  • Orunsi, NA & Trinci, APJ (1985) Growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin B. Microbios 43: 17–30

    Google Scholar 

  • Patton, RS & Chandler, PT (1975) In vivo digestibility evaluation of chitinous material. J Dairy Sci 58: 397–403

    Google Scholar 

  • Payne, S, Ahl, P, Moyer, M, Harper, A, Beck, J, Meins, F & Ryals, J (1990) Isolation of complementary DNA clones encoding pathogeneis-related proteins P and Q, two acid chitinases from tobacco. Proc Nat Acad Sci USA 87: 98–102

    Google Scholar 

  • Peberdy, J (1983) Genetic recombination in fungi following protoplast fusion and transformation. In: Smith, JE (Ed) Fungal Differentiation (pp 559–581). Dekker, New York

    Google Scholar 

  • Pel, R & Gottschal, JC (1986a) Chitinolytic communities from an anaerobic estuarine environment, In Muzzarelli, RAA, Jeuniaux, C & Gooday, GW (Eds) Chitin in Nature and Technology (pp 539–546). Plenum, New York

    Google Scholar 

  • (1986b) Stimulation of anaerobic chitin degradation in mixed cultures, Antonie van Leewenhoek 52: 359–360

    Google Scholar 

  • (1989) Interspecies interaction based on transfer of a thioredoxin-like compound in anaerobic chitin-degrading mixed cultures. FEMS Microbiol Ecol 62: 349–358

    Google Scholar 

  • Pel, R, Wessels, G, Aalfs, H & Gottschal, JC (1989) Chitin degradation by Clostridium sp. strain 9.1 in mixed cultures with saccharolytic and sulphate-reducing bacteria. FEMS Microbiol Ecol 62: 191–200

    Google Scholar 

  • Pel, R, Wijngaard, AjVan Den, Epping, E & Gottschal, JC (1990) Comparison of the chitinolytic properties of Clostridium sp. strain 9.1 and a chitin degrading bacterium from the intestinal tract of the plaice Pleuronectes platessa (L.). J Gen Microbiol 136: 695–704

    Google Scholar 

  • Pelletier, A & Sygusch, J (1990) Purification and characterization of three chitosanase activities from Bacillus megaterium P1. Appl Environ Microbiol 56: 844–848

    Google Scholar 

  • Pelletier, A, Lemire, I, Sygusch, J, Chornet, E & Overend, RP (1990) Chitin/chitosan transformation by thermo-mechano-chemical treatment including characterization by enzymatic depolymerisation. Biotech Bioeng 36: 310–315

    Google Scholar 

  • Peter, MG, Kegel, G & Keller, R (1986) Structural studies on sclerotized insect cuticle. In: Muzzarelli, R, Jeuniaux, C, Gooday, GW (Eds) Chitin in Nature and Technology (pp 21–28). Plenum Press, New York

    Google Scholar 

  • Petz, W, Foissner, W, Wirnsberger, E, Kruatgartner, Wd & Adam, H (1986) Mycophagy, a new feeding strategy in autochthonous ciliates. Naturwissenschaften 73: S.560–561

    Google Scholar 

  • Pope, AMS & Davies, DAL (1979) The influence of carbohydrases on the growth of fungal pathogens in vitro and in vivo. Postgrad Med J 55: 674–676

    Google Scholar 

  • Poulicek, M, Voss-Foucart, MF & Jeuniaux, C (1986) Chitinoproteic complexes and mineralization in mollusk skeletal structures. In: Muzzarelli, R, Jeuniaux, C, Gooday, GW (Eds) Chitin in Nature and Technology (pp 7–12). Plenum Press, New York

    Google Scholar 

  • Reid, JD & Ogrydziak, DM (1981) Chitinase-overproducing mutant of Serratia marcescens. Appl Environ Microbiol 41: 664–669

    Google Scholar 

  • Reichardt, W, Gunn, B and Colwell, RR (1983) Ecology and taxonomy of chitinoclastic Cytophaga and related chitin-degrading bacteria isolated from an estuary, Microb Ecol 9: 273–294

    Google Scholar 

  • Reisert, PS & Fuller, MS (1962) Decomposition of chitin by Chytridiomyces species. Mycologia 54: 647–657

    Google Scholar 

  • Reyes, F, Perez-Leblic, MI, Martinez, MJ & Lahoz, R (1984) Protoplast production from filamentous fungi with their own autolytic enzymes. FEMS Microbiol Lett 24: 281–283

    Google Scholar 

  • Reyes, F, Lahoz, R, Martinez, MJ & Alfonso, C (1985) Chitosanases in the autolysis of Mucor rouxii. Mycopathologia 89: 181–187

    Google Scholar 

  • Robbins, PW, Albright, C & Benfield, B (1988) Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli. J Biol Chem 263: 443–447

    Google Scholar 

  • Roberts, RL & Cabib, E (1982) Serratia marcescens chitinase: one-step purification and use for the determination of chitin. Anal Biochem 127: 402–412

    Google Scholar 

  • Roberts, WK & Selitrennikoff, CP (1988) Plant and bacterial chitinases differ in antifungal activity. J Gen Microbiol 134: 169–176

    Google Scholar 

  • Sakuda, S, Isogai, A, Matsumoto, S & Suzuki, A (1987a) Search for microbial insect growth regulators II. Allosamidin, a novel insect chitinase inhibitor. J Antibiot 40: 296–300

    Google Scholar 

  • Sakuda, S, Isogai, A, Makita, T, Matsumoto, S, Koseki, K, Kodama, H & Suzuki, A (1987b) Structures of Allosamidins, novel insect chitinase inhibitors, produced by actinomycetes. Agric Biol Chem 51: 3251–3259

    Google Scholar 

  • Sakuda, A, Nishimoto, Y, Ohi, M, Watanabe, M, Takayama, T, Isogai, A & Yamada, Y (1990) Effects of demethylallosamidin, a potent yeast chitinase inhibitor, on the cell division of yeast. Agric Biol Chem 54: 1333–1335

    Google Scholar 

  • Sassen, MMA (1965) Breakdown of the plant cell wall during the cell fusion process. Acta Bot Neerlandica 14: 165–196

    Google Scholar 

  • Seki, H & Taga, N (1965) Microbial studies on the decomposition of chitin in marine environment-VI. Chitinoclastic bacteria in the digestive tract of whales from the Antarctic Ocean. J Oceanogr. Soc Japan 20: 272–277

    Google Scholar 

  • Shapira, R, Ordentlich, A, Chet, I & Oppenheim, AB (1989) Control of plant diseases by chitinase expressed from cloned DNA in Escherichia coli. Phytopathology 79: 1246–1249

    Google Scholar 

  • Shinshi, H, Beuhaus, J, Ryals, J & Meins, F (1990) Structure of a tobacco chitinase gene: evidence that different chitinase genes can arise by a transposition of sequences encoding a cysteine-rich domain. Plant Mol Biol 14: 357–368

    Google Scholar 

  • Sietsma, JH, Vermeulen, CA & Wessels, JGH (1986) The role of chitin in hyphal miorphogenesis. In Muzzarelli, R, Jeuniaux, C & Gooday, GW (Eds) Chitin in Nature and Technology (pp 6369). Plenum Press, New York

    Google Scholar 

  • Sivan, A & Chet, I (1989) Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. J Gen Microbiol 135: 675–682

    Google Scholar 

  • Skjak-Braek, G, Anthosen, T & Sandford, P (1989) Chitin and Chitosan, Elsevier, London

    Google Scholar 

  • Smith, RJ & Grula, EA (1983) Chitinase is an inducible enzyme in Beauvaria bassiana. I Invertebr Path 42 319–326

    Google Scholar 

  • Bneh, B (1981) The use of rhizophere chitinolytic bacteria for biological control. Phytopath Zeitscrift 100: 251–256

    Google Scholar 

  • Soderhall, K & Unestam, T (1975) Properties of extracellular enzymes from Aphanomyces astaci and their relevance in the penetration process of crayfish cuticle. Physiol Plant 35: 140–146

    Google Scholar 

  • Somers, PJB, Yao, RC, Doolin, LR, McGowan, MJ, Fakuda, DS & Mynderse, JS (1987) Method for the detection and quantitation of chitinase inhibitors in fermentation broths: Isolation and insect life cycle. Effect of A82516. J Antibiot 40: 1751–1756

    Google Scholar 

  • Soto-Gil, RW & Zyskind, JW (1984) Cloning of Vibrio harveyi chitinase and chitobiase gene in Escherichia coli. In: Zikakis, JP (Ed) Chitin, Chitosan and Related Enzymes (pp 209–223). Academic Press, Orlando

    Google Scholar 

  • Srivastava, AK, Defago, G & Boller, T (1985) Secretion of chitinase by Aphanocladium album, a hyperparsite of wheat. Experientia 41: 1612–1613

    Google Scholar 

  • St Leger, RJ, Cooper, RM & Charnley, AK (1986) Cuticle-degrading enzymes of entomopathogenic fungi: regulation of production of chitinolytic enzymes. J Gen Microbiol 132: 1509–1517

    Google Scholar 

  • Surarit, R, Gopel, PK & Shepherd, MG (1988) Evidence for a glycosidic linkage between chitin and glucan in the cell wall of Candida albicans. J Gen Microbiol 134: 1723–1730

    Google Scholar 

  • Suslow TV & Jones J (1988) Chitinase-producing bacteria. US Patent No 4751081

  • Suzuki, K, Mikami, T, Okawa, Y, Tokora, A, Suzuki, S & Suzuki, M (1986) Antitumour effect of hexa-N-acetylchitohexase and chitohexaose. Carb Res 151: 403–408

    Google Scholar 

  • Takiguchi, Y & Shimahara, K (1988) N,N-Diacetylchitobiose production from chitin by Vibrio anguillarum strain E-383a. Lett Appl Microbiol 6 129–131

    Google Scholar 

  • (1989) Isolation and identification of a thermophilic bacterium producing N,N-diacetylchitobiose from chitin. Agric Biol Chem 53: 1537–1541

    Google Scholar 

  • Taylor, JL, Jones, JDG, Sandler, S, Mueller, GM, Bedbrook, J & Dunsmuir, P (1987) Optimizing the expression of chimeric genes in plant cells. Mol Gen Genet 210: 572–577

    Google Scholar 

  • Tracey, MV (1955) Chitinase in some Basidiomycetes. Biochem J 61: 579–589

    Google Scholar 

  • Usai, T, Hayashi, Y, Nanjo, F, Sakai, K & Ishido, Y (1987) Transglycosylation reaction of a chitinase purified from Nocardia orientalis. Biochem Biophys Acta 923: 302–309

    Google Scholar 

  • Usai, T, Matsui, M & Isobe, K (1990) Enzymic synthesis of useful chito-oligosaccharides utilizing transglycosylation by chitinolytic enzymes in buffer containing ammonium sulfate. Carb Res 202: 65–78

    Google Scholar 

  • Walker, AN, Garner, RE & Horst, MN (1990) Immunocytochemical detection of chitin in Pneumocystis carinii. Infect Immun 58: 412–415

    Google Scholar 

  • Wessels, JGH (1988) A steady state model for apical wall growth. Acta Bot Neerl 37: 3–16

    Google Scholar 

  • Wolkin, RH & Pate, JKL (1985) Selection for nonadherent or nonhydrophobic mutants co-selects for non-spreading mutants of Cytophage johnsonae and other gliding bacteria. J Gen Microbiol 131: 7737–750

    Google Scholar 

  • Wortman, AT, Somerville, CC & Colwell, RR (1986) Chitinase determinants of Vibrio vulnificus: Gene cloning and applications of a chitinase probe. Appl Environ Microbiol 52: 142–145

    Google Scholar 

  • Yabuki, M, Kasai, Y, Ando, A & Fujii, T (1984) Rapid method for converting fungal cells into protoplasts with a high regeneration frequency. Exp Mycol 8: 386–390

    Google Scholar 

  • Zarain-Herzberg, A & Arroya-Begovich, A (1983) Chitinolytic activity from Neurospora crassa. J Gen Microbiol 129: 3319–3326

    Google Scholar 

  • Zhloba, NM, Tiunova, NA & Sidorova II (1980) extracellular hydrolytic enzymes of mycophilic fungi. Mikol Fitopatol 14: 496–499

    Google Scholar 

  • Zikakis, J (1984) Chitin, chitosan and Related Enzymes. Academic Press, Orlando

    Google Scholar 

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  1. Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, AB9 1AS, Aberdeen, UK

    Graham W. Gooday

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  1. Graham W. Gooday
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Gooday, G.W. Physiology of microbial degradation of chitin and chitosan. Biodegradation 1, 177–190 (1990). https://doi.org/10.1007/BF00058835

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  • DOI: https://doi.org/10.1007/BF00058835

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