Abstract
Callus was induced from seeds of Puccinellia distans (L.) Parl. on MS medium supplemented with 2 mgl-1 2,4-dichlorophenoxyacetic acid and 0.5 mgl-1 kinetin. Morphogenesis initiation was achieved during subculture on medium containing 0.1 mgl-1 2,4-D. From the point of morphogenetic capacity, 3 types of callus were selected. High frequency of plant regeneration was obtained by selection of embryogenic type of callus, and culture on N6 medium and N6 medium supplemented with kinetin (5–10 mgl-1), or kinetin (2 mgl-1) and IAA (0.5 mgl-1). A high ratio of albinos among regenerants was observed.
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References
Vasil JK (1983) Regeneration of plants from single cells of cereals and grasses. In: Lurgnin PF, Kleinhofs A (Eds) Genetic Engineering in Eukaryotes (p 233–252) Plenum Publishing Co, New York
Vasil V, Vasil JK (1984) Induction and maintenance of embryogenic callus cultures of Gramineae. In: Vasil JK (Ed) Cell Culture and Somatic Cell Genetics of Plants (p 36–42) Academic Press, New York
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Chu CC, Wang CC, Sun CS, Hus C, Yin KC, Chu CY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sinica 18: 659–668
Nabors MW, Heyser JW, Dykes TA, DeMott KJ (1983) Long duration, high-frequency plant regeneration from cereal tissue cultures. Planta 157: 385–391
Orton TJ (1979) A quantitative analysis of growth and regeneration from tissue cultures of Hordeum vulgare, H. jubatum and their interspecific hybrids. Env Exp Bot 19: 319–335
Evans DA, Sharp WR, Flich CE (1981) Growth and behaviour of cell cultures: embryogenesis and organogenesis. In: Thorpe TA (Ed) Plant Tissue Culture Methods and Applications in Agriculture (p 45–114) Academic Press, Inc
Fladung M, Hesselbach J (1986) Callus induction and plant regeneration in Panicum bisulcatum and Panicum millioides. Plant Cell Rep 3: 169–173
Heszky LE (1973) Investigation at the early stage of embryogenesis into the development of the adventive embryo organized from a cell of the callus tissue in Daucus carota L. Acta Bot 18: 303–305
Heszky LE (1975) Possible ways of morphogenesis in higher plants tissue cultures. Acta Agron 24: 123–141
Abe T, Futsuhara Y (1986) Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theor Appl Genet 72: 3–10
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Binh, D.Q., Heszky, L.E., Gyulai, G. et al. Plant regeneration from callus of Puccinellia distans (L.) Parl. Plant Cell Tiss Organ Cult 18, 195–200 (1989). https://doi.org/10.1007/BF00047745
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DOI: https://doi.org/10.1007/BF00047745