Abstract
Callus growth and the production of anthocyanins were sustained on the salts and vitamins of Murashige and Skoog. Callus growth was stimulated at a concentration of 8–32 μM α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-d). Benzyladenine (BA) and zeatin at 8 μM inhibited callus growth whereas isopentenyladenine (iP) stimulated callus growth. NAA repressed anthocyanin production with an increase in NAA from 8–32 μM. Anthocyanin synthesis was promoted by an increase in 2,4-d from 0.5 to 2 μM and decreased thereafter up to a concentration 32 μM 2,4-d. A concentration of 8 μM BA, thidiazuron and zeatin, respectively stimulated pigment production. Sucrose stimulated callus growth at 60 mM and pigment production at 120–360 mM.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- iP:
-
isopentenyladenine
- TZ:
-
thidiazuron-N-phenyl-N′-1,2,3-thiadiazol-5-yl-urea
- Bu-HCl:
-
Butanol-2N HCl
- BAW:
-
Butanol-acetic acid-water
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Meyer, H.J., Van Staden, J. The in vitro production of an anthocyanin from callus cultures of Oxalis linearis . Plant Cell Tiss Organ Cult 40, 55–58 (1995). https://doi.org/10.1007/BF00041119
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DOI: https://doi.org/10.1007/BF00041119