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The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues

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Abstract

The octopine synthase (ocs or ocs-like) element has been previously reported to be responsive to the plant hormones, auxin, salicylic acid, and methyl jasmonate. Using transient assays with carrot protoplasts, we have demonstrated that an ocs element from the soybean auxin-inducible GH2/4 promoter is not only activated by strong auxins (i.e, 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, α-naphthalene acetic acid) and salicylic acid, but also by weak auxin analogues (β-naphthalene acetic acid), inactive auxin analogs (i.e., 2,3-dichlorophenoxyacetic acid, 2,4,6-trichlorophenoxyacetic acid), and inactive salicylic acid analogs (3-hydroxybenzoic acid and 4-hydroxybenzoic acid). Our results indicate that the ocs element in the GH2/4 promoter is not selectively induced by plant hormones and might function similarly to tandem AP-1 sites in some animal glutathione S-transferase (GST) genes. The ocs element, like the AP-1 sites in animal GST promoters, may be induced not only by certain hormones but also by some non-hormonal stress-inducing or electrophilic agents.

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Abbreviations

GST:

glutathione S-transferase

MUG:

4-methyl-umbelliferyl-glucuronide

GUS:

β-glucuronidase

2,4-D:

2,4-dichlorophenoxyacetic acid

2,3-D:

2,3-dichlorophenoxyacetic acid

2,4,5-T:

2,4,5-trichlorophenoxyacetic acid

2,4,6-T:

2,4,6-trichlorophenoxyacetic acid

NAA:

naphthalene acetic acid

SA:

salicylic acid

SARE :

putative salicylic acid-responsive element

BA:

benzoic acid

UTR:

untranslated region

nos :

nopaline synthase

ocs :

octopine synthase

mas :

mannopine synthase

ocs :

element-(−)46 CaMV 35S promoter-GUS reporter gene: the ocs element fused to a minimal −46 cauliflower mosaic virus 35S promoter fused to a GUS reporter gene with a 3′ nos untranslated region

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Authors and Affiliations

  1. Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, 65211, Columbia, MO, USA

    Tim Ulmasov, Gretchen Hagen & Tom Guilfoyle

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  1. Tim Ulmasov
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  2. Gretchen Hagen
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  3. Tom Guilfoyle
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Ulmasov, T., Hagen, G. & Guilfoyle, T. The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues. Plant Mol Biol 26, 1055–1064 (1994). https://doi.org/10.1007/BF00040688

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  • DOI: https://doi.org/10.1007/BF00040688

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