Abstract
Carrageenan analyses were conducted on vegetative female clones of Chondrus crispus that were cultured to provide tissues with differing growth rates. Tissue dry weights increased from apex to base of fronds. Total carrageenan contents were lower in apical 1 to 2 cm segments than elsewhere in the frond, except when the alga was grown at high photon irradiances. Clone 373A contained more carrageenan than clone G8. The proportion of 0.3 M KCl-soluble polymers in the total native carrageenans varied from 44 to 92%, being highest in older tissues of fronds cultured at high photon irradiances. The apical 1 cm segments contained less KCl-soluble carrageenans than other tissues from the corresponding fronds. The KCl-soluble carrageenans, when alkali-modified and refractionated, afforded the expected kappa-iota carrageenan in > 79% yields. The remainder consisted of a polymer containing 23.1% SO3Na and 8.4% 3,6-anhydrogalactose. Lambda carrageenan was not detected. Variations in carrageenan distribution between the apical region and other parts of the frond may reflect the increasing influence of medullary tissue developed as the immature cells differentiate.
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Bird, K., K. Pendoley & F. Koehn, 1990. Variability in agar gel behavior and chemistry as affected by algal growth under different environmental conditions. In V. Crescenzi, I. C. M. Dea & S. S. Stivala (eds), Biomedical and Biotechnological Advances. Gordon and Breach, Science Publishers, New York: in press.
Bremond, C. de L., M. Quillet & M. Bremond, 1987. λ-carrageenan in the gametophytes of Chondrus crispus. Phytochemistry 26: 1705–1707.
Cheney, D., A. C. Mathieson & D. Schubert, 1981. The application of genetic improvement techniques to seaweed cultivation: I. Strain selection in the carrageenophyte Chondrus crispus. Proc. int. Seaweed Symp. 10: 559–567.
Chopin, T., 1986. The red alga Chondrus crispus Stackhouse (Irish moss) and carrageenans — a review. Can. Tech. Rept. Fish. Aquat. Sci. 1514: 69 pp.
Craigie, J. S. & C. Leigh, 1978. Carrageenans and agars. In J. A. Hellebust & J. S. Craigie (eds), Handbook of Phycological Methods: Physiological and Biochemical Methods. Cambridge University Press, New York: 109–131.
Craigie, J. S., 1990a. Irish moss cultivation — some reflections. In C. Yarish, C. Penniman & M. VanPatten (eds), Economically Important Marine Plants of the Atlantic: Their Biology and Cultivation. The University of Connecticut Press, Avery Point (CT): 31–52.
Craigie, J. S., 1990b. Cell walls. In R. G. Sheath & K. M. Cole (eds), The Biology of Red Algae. Cambridge University Press, New York: in press.
Craigie, J. S. & P. F. Shacklock, 1989. Culture of Irish moss. In A. D. Boghen (ed.), Cold Water Aquaculture in Atlantic Canada. The Canadian Institute for Research on Regional Development, Moncton (N.B.): 243–270.
Gordon-Mills, E. M. & E. L. McCandless, 1975. Carrageenans in the cell walls of Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody. Phycologia 14: 275–281.
Harvey, M. J. & J. McLachlan (eds), 1973. Chondrus crispus. Proc. N.S. Inst. Sci. Suppl. 217: 155 pp.
Millipore, 1987. Waters Publications 91046, Rev. C. 1987 (11/85, 8/84).
Taylor, A. R. A. & L. C.-M. Chen, 1990.Chondrus Stackhouse. In I. Akatsuka (ed.), Biology of Economic Seaweeds. Dr. W. Junk Publishers, Dordrecht: in press.
Tian, M-X., Z-C. Wen & C. Y. Wu, 1988. Effects of tissue age and season on the content and composition of carrageenan in Eucheuma species. In T. Stadler, J. Mollion, M-C. Verdus, Y. Karamanos, H. Morvan & D. Christian (eds), Algal Biotechnology. Elsevier Applied Science, London: 383–392.
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Rivera-Carro, H., Craigie, J.S. & Shacklock, P.F. Influence of tissue source and growth rates on dry weight and carrageenan composition of Chondrus crispus (Gigartinales, Rhodophyta). Hydrobiologia 204, 533–538 (1990). https://doi.org/10.1007/BF00040282
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DOI: https://doi.org/10.1007/BF00040282