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Characterization of a predominantly pistil-expressed gene encoding a γ-thionin-like protein of Petunia inflata

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Abstract

We isolated a cDNA clone from a pistil cDNA library of Petunia inflata which encodes a protein, PPT, with sequence similarity to γ-thionins. Characterization of a genomic clone containing a PPT gene revealed the presence of a single intron. Northern analysis revealed that the PPT gene was predominantly expressed in the pistil during all stages of flower development. Since thionins have been implicated in plant defense against pathogens, PPT may play a role similar to that of other defense-related proteins found in the pistil, defending the pistil against pathogen infection.

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Author notes

  1. Anuradha Singh

    Present address: Department of Biology, Utah State University, 84323, Logan, UT, USA

Authors and Affiliations

  1. Graduate Program in Plant Physiology, The Pennsylvania State University, 16802, University Park, PA, USA

    Balasulojini Karunanandaa & Teh-hui Kao

  2. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 16802, University Park, PA, USA

    Anuradha Singh & Teh-hui Kao

Authors
  1. Balasulojini Karunanandaa
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  2. Anuradha Singh
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  3. Teh-hui Kao
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Karunanandaa, B., Singh, A. & Kao, Th. Characterization of a predominantly pistil-expressed gene encoding a γ-thionin-like protein of Petunia inflata . Plant Mol Biol 26, 459–464 (1994). https://doi.org/10.1007/BF00039555

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  • DOI: https://doi.org/10.1007/BF00039555

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